ISSN:
1476-5535
Schlagwort(e):
Esterase screening test
;
Esterase production
;
B. subtilis esterase
;
Temperature profile
;
pH profile
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
Notizen:
Summary The conventional esterase plate-screening technique has been modified in order to obtain a better differentiation of high producing strains. A short exposure to Lugol's iodine solution after colony growth enhanced the contrast between the precipitation halo and the background. Batch cultures of a selected strain characterized as belonging to theBacillus subtilis group showed a high level of extracellular activity at pH 6.6 to 8.0 and 35°C. In crude extracts the optimum enzymatic activity was obtained at 35°C and pH 7.0.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF01569761
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