ISSN:
1432-2242
Schlagwort(e):
κ-casein encoding gene
;
DNA regulatory sequences
;
Comparative DNA sequencing DNA variants
;
Bovine
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract In order to identify DNA variants within the 5′-flanking region of the bovine κ-casein (κCn)-encoding gene, this area of the gene from 13 cows belonging to seven breeds (Holstein Friesian, Brown Swiss, German Simmental, Jersey, Galloway, Scottish Highland and Ceylon Dwarf Zebu) was analysed. For each individual, about 1 kb of the 5′-flanking region including exon I was amplified by polymerase chain reaction (PCR). The biotinylated PCR product was immobilized on magnetic beads followed by direct bidirectional sequencing using an automated DNA sequencer. Fifteen DNA variants were identified, some of which are located within potential regulatory sites and possibly involved in the expression of the κ-casein encoding gene.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00226992
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