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  • 1
    ISSN: 1432-0533
    Keywords: Neuropathy ; Immunotactoid ; Endoneurial deposits ; Monoclonal gammopathy ; Skin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An 85-year-old man with a 2-year history of progressive lower limb weakness and paresthesia was found to have an IgG kappa monoclonal gammopathy of undetermined significance (mgus). Clinical and electrophysiological studies revealed a severe distal bilateral symmetrical polyneuropathy. a sural nerve biopsy showed extensive nerve fibre loss with the deposition of large amounts of amorphous material throughout the endoneurium. Electron microscopy showed the deposits to be composed of microtubular structures which were located diffusely throughout the endoneurium. The deposits were also located within the lumina of the vasa nervorum, some of which were undergoing disintegration and rupture with release of the proteinaceous material into the endoneurium. The regions of the nerve in which they appeared most numerous showed more severe nerve fibre damage than other areas. These microtubular structures were also observed in disintegrating vessels and adjacent endoneurium. On immunohistochemistry they stained with antibody to IgG. Identical deposits were found in the dermis in which there was a leucocytoclastic vasculitis. Located in linear arrays within the axons of myelinated and unmyelinated fibres were highly organised tubular structures resembling immunotactoids. Identification of immunotactoidlike structures within the nerve is unique and may be another mechanism by which monoclonal proteins can induce nerve fibre injury.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 98 (1994), S. 13144-13150 
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 8 (1992), S. 0 
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. The accuracy of assays based on galactosidase and the enzyme-linked immunosorbent assay specific to Thanatephorus cucumeris were compared with techniques based on soil dilution plating and baiting in sterilized field soil. Although soil dilution plating is reasonably quantitative, it requires substantial time, material and labour. Plant baits gave inconsistent results in the estimation of T. cucumeris populations in the soil. Enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies is suitable for detecting the presence of a range of anastomosis groups (AGs) of 71 cucumeris in soil samples, but more quantitative applications seem to be limited to a very narrow range of concentrations of the fungus (0–10 μg/g). Monoclonal antibody ELISA could be used if the soil samples are routinely further diluted, provided the range of concentrations is uniformly low. An assay of β-galactosidase permits estimation of a more adequate range of concentrations (0–500 μg/g) and may be used in defined experiments using uninoculated soil samples.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 23 (1993), S. 0 
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Adult fleas, spent and unspent culture media were extracted and the radio-atlergosor-bent test (RAST) performed with sera of 48 cat flea skin test-positive individuals from the Tampa Bay area of Florida. Sixteen sera (33.6%) had a positive RAST to the cat flea extract prepared in our laboratory [1.7-11.4% of the total counts (TC) added]. Six of the 16 sera (12.5%) also contained specific IgE to allergens in thespent medium (0.8-3.3% TC). The allergen composition and strength were studied by RAST inhibition of two commercial cat flea extracts and compared with our in-house flea extract. The results demonstrated similar allergen compositions and different potencies. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of the in-house flea extract showed several Coomassie blue-stained bands (10–85 kD). SDS-PAGE immunoblols revealed five IgE-binding bands at 34, 35, 39, 54 and 60 kD. Flea allergens were quantified in eight house dust samples using RAST inhibition assays and expressed as RAST inhibition units; five of these samples contained detectable levels. Cat flea allergens may contribute to the allergenicity of house dust in areas of heavy llea infestation.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 41 (1992), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The incidence of potato pathogens on healthy roots of micropropagated (MP) and seed tuber (ST) plants was examined on successive dates during the growing season in two field experiments. Microplants were grown in a glasshouse for 4–5 weeks in perlite or peal-based substrates, and exposed or not to natural inoculum before planting in the field. The seed tubers originated from stocks of visually clean or moderately blemished tubers and were surface-sterilized or not before planting. Polyscytalum pustulans and Helminthosporium solani only infected roots of ST plants and inoculated MP plants. The incidence of P. pustulans was affected by seed tuber-borne inoculum and, in I year, by the substrate. H. solani was detected infrequently on roots. Rhizoctonia solani was present at low frequencies in most root samples, and more ST than MP plant roots were colonized; there were no substrate effects. In 1 year, increased inoculum levels increased root infection, but only in MP roots. Colletotrichum coccodes occurred at high frequencies and was most common in roots of ST plants. Progeny tubers showed some treatment effects when tested in September and after storage for 6 months, but there were no consistent relationships between root and progeny tuber infection.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 41 (1992), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The powdery mildew Erysiphe pisi var. pisi, complete with its teleomorph, has been observed on faba beans (Vicia faba cultivars) for the first time in Britain. It appeared in a glasshouse on Syrian lines of fodder bean and English cultivars of broad bean. It also infected tare (Vicia sativa) and sweet pea (Lathyrus odoratus), but not edible pea (Pisum sativum), ornamental lupin (Lupinus polyphyllus), French bean (Phaseolus vulgaris) or sainfoin (Onobrychis viciaefolia). Inoculations in a glasshouse showed that another strain of E. pisi var. pisi found on outdoor everlasting pea (Lathyrus latifolius) was also capable of affecting faba bean, and was distinguished by its ability to infect edible pea. A third strain found on outdoor ornamental lupin infected only lupin. E. pisi var. pisi was shown to be morphologically and pathogenically distinct from the Erysiphe sp. newly affecting tomatoes in Britain.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY : American Institute of Physics (AIP)
    Physics of Fluids 4 (1992), S. 1230-1244 
    ISSN: 1089-7666
    Source: AIP Digital Archive
    Topics: Physics
    Notes: A turbulent-molecular-mixing closure for passive scalar mixing is derived based on the theory of diffusion in layerlike lamellar structures. The closure is formulated in terms of the Fokker–Planck (FP) equation (or an equivalent stochastic differential equation), and is to be employed in conjunction with the probability density function (pdf) balance equation appearing in the pdf methods for modeling turbulent reactive flows. Like the mapping closure, the FP closure predicts a limiting Gaussian pdf for the passive scalar concentration in isotropic turbulence. In addition, the FP closure models the joint pdf of the scalar concentration and the scalar gradient and thus predicts the scalar dissipation rate. The closure predictions for the scalar rms concentration, the marginal pdf, and the joint pdf as well as other relevant statistics have been studied by Monte Carlo simulation. The shape and temporal evolution of the marginal pdf for the scalar concentration compare favorably with published results found from direct numerical simulations and from the mapping closure. Finally, unlike the mapping closure, the FP closure is shown to easily extend to the case of multiple scalar mixing.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Physics of Fluids 6 (1994), S. 334-348 
    ISSN: 1089-7666
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The joint scalar, scalar gradient probability density function (PDF) of an inert nonpremixed scalar diffusing in a one-dimensional system of random-sized lamellas is investigated by numerical simulation. The form of the scalar PDF, at a given RMS value, is nearly identical to that predicted by direct numerical simulation (DNS) of scalar mixing in isotropic turbulence and the mapping closure, and the moments of both the scalar and the scalar gradient suggest that their limiting marginal PDF are Gaussian. The joint scalar, scalar gradient PDF is found to be restricted to a bounded region in the scalar–scalar gradient plane, whose form is independent of the initial mixing ratio. These results are incorporated into the Fokker–Planck (FP) model for the joint scalar, scalar gradient PDF, and the improved model shows good agreement with numerical simulation data. An extension of the FP model that includes random stretching of the scalar gradient in isotropic turbulence is formulated.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    BJOG 97 (1990), S. 0 
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    BJOG 97 (1990), S. 0 
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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