ISSN:
1471-4159
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
The parathyroid hormone-related peptide (PTHrP) gene has been reported to be subject to a wide variety of physiological and pharmacological controls. Two distinct patterns of PTHrP mRNA response have been recognized, one characterized by a prolonged or plateau response lasting many hours to days and the second characterized by rapid induction-deinduction kinetics and lasting 1 to several hours. The kinetics of the second pattern are similar to those displayed by primary response genes like nuclear protooncogenes, cytokines, and growth factors. In GH4C1, rat pituitary cells, 17β-estradiol induced a rapid and transient increase in PTHrP mRNA expression, with a peak response at 1–2 h. This response appeared to be due to a rapid and transient burst in gene transcription, which by runoff analysis was maximal at 20–40 min and declined thereafter. PTHrP mRNA half-life was 30 min in these cells and was unaltered by estradiol. Cy-cloheximide did not block the 17β-estradiol-induced response but rather prolonged it, and runoff analysis revealed that this effect was due to a prolongation or persistence of PTHrP gene transcription. These findings suggest that the transient nature of the native response reflects the effects of an estrogen-inducible represser. All of these features are characteristic of a prototypical primary response gene.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1046/j.1471-4159.1994.62041239.x
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