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  • 1
    ISSN: 1520-5827
    Quelle: ACS Legacy Archives
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
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  • 2
    Digitale Medien
    Digitale Medien
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 65 (1994), S. 1317-1319 
    ISSN: 1077-3118
    Quelle: AIP Digital Archive
    Thema: Physik
    Notizen: Spatially resolved concentrations of excited atomic hydrogen (n=2), generated from 13.56 MHz RF hydrocarbon discharges, are determined during film formation using intracavity laser spectroscopy. Self-bias voltages and pressures of methane (25 and 100 mTorr) and benzene (25 mTorr) are selected so that diamondlike carbon films are deposited. In all cases, the minimum concentration occurs at the power electrode. Benzene produces the least amount of excited atomic hydrogen overall. The results may have implications concerning the proposed impact induced fragmentation of hydrocarbon molecules during film growth.
    Materialart: Digitale Medien
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  • 3
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    Unbekannt
    Chapel Hill, N.C. : Periodicals Archive Online (PAO)
    Social Forces. 3:1/4 (1924/1925) 769 
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  • 4
    ISSN: 1662-9752
    Quelle: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Thema: Maschinenbau
    Materialart: Digitale Medien
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  • 5
    facet.materialart.
    Unbekannt
    Oxford : Periodicals Archive Online (PAO)
    Economic Inquiry. 28:3 (1990:July) 604 
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  • 6
    Digitale Medien
    Digitale Medien
    Springer
    European spine journal 3 (1994), S. 32-38 
    ISSN: 1432-0932
    Schlagwort(e): Anterior lumbar fusion ; Interbody fusion ; Allograft bone ; Low-back pain
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary This is a radiographic report of 40 patients (20 men, 20 women) who underwent anterior lumbar interbody fusions (73 levels) utilizing a “hybrid” interbody graft composed of femoral cortical allograft (FCA) bone and iliac crest cancellous autograft bone. The average age at surgery was 38 years (range 17–64 years), and follow-up averaged 1.4 years (range 1.0–2.4 years). Nineteen of the patients had undergone previous lumbar surgery. Thirty-two patients (63 levels) underwent anterior fusion combined with some type of posterior fixation, and eight patients (10 levels) had no posterior fixation. Types of posterior fixation included: for 20 patients (36 levels) Steffee variable screw placement fixation, for 10 patients (23 levels) translaminar facet screws (TFS), for 1 patient (3 levels) Knodt rods and for 1 patient (1 level) facet screws. Based on the persistence of lucent lines at the graft-host interface, three patients (one level each) were felt to have non-unions at their latest follow-ups at 1.4, 1.5 and 2.0 years, respectively. Two of these patients had no posterior fixation, and the other had TFS fixation. The overall fusion rate was 96% (70 of 73 levels). The fusion rate for all levels treated with posterior fixation was 98% compared with 75% for those without fixation. Intervertebral disc heights (IVDH) were measured on all films and corrected for magnification with computer assistance. On average, the IVDH was increased postoperatively but returned to preoperative values at follow-up. IVDH loss was independent of the type of instrumentation used. No complications arose from the use of the hybrid graft. Incorporation of the allograft portion of the graft is slow and was felt to be complete in only 7 of the 73 levels at follow-up. We conclude that the hybrid interbody graft technique is a safe and reliable method for performing anterior lumbar interbody fusions and should be combined with some type of posterior fixation. Long-term follow-up will be required to assess the behaviour of the allograft until incorporation is complete.
    Materialart: Digitale Medien
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  • 7
    Digitale Medien
    Digitale Medien
    Springer
    European spine journal 3 (1994), S. 28-31 
    ISSN: 1432-0932
    Schlagwort(e): Discography ; Delayed pain onset ; Incomplete annular tears ; Anterior spinal tenderness
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The delayed onset of symptomatic pain following lumbar discography (with no immediate pain response) is described in six patients, five with a minimum 2-year follow-up. It is usually seen in patients with nearly normal disc morphology who have incomplete or discrete annular tears that are not filled at the time of injection. Later (2–12 h in this study), dye leakage occurs through these lesions, thereby precipitating the discogenic pain This phenomenon may be missed and is probably more common than previously believed due to early discharge from the hospital, the patient expecting discomfort after the invasive study (hence no complaint is made), and the clinician being unaware of this delayed symptom, thereby not asking about it in follow-up. Close patient questioning regarding a delayed onset of symptomatic pain after discography is, therefore, an essential element in diagnostic information following this study.
    Materialart: Digitale Medien
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  • 8
    ISSN: 1432-2013
    Schlagwort(e): Small-intestinal crypts ; Intestinal secretion ; Cystic fibrosis ; Chloride conductance ; DIDS ; Glibenclamide ; Mouse
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The cellular volume of crypts isolated from 2- to 3-week-old mouse small intestine has been measured to assess the capacity of the epithelial cells to respond to secretagogues. Vasoactive intestinal polypeptide (VIP) or carbachol, respectively cAMP- and calcium-mediated secretagogues, produced a reduction crypt volume attributed to KCl loss through channels activated by the agonists. Consistent with the participation of separate chloride channels, 4,4′-diisothiocyanatostilbene-2,2′-disulphonic acid (DIDS) blocked the carbacholbut not the VIP-induced volume decrease, whilst gl- ibenclamide abolished the VIP effect without affecting the carbachol-induced volume decrease. Animals homozygous for a disrupted cftr gene, introduced by gene targeting, were also used as the source for crypt isolation. In these CFTR(-/-) crypts, VIP failed to elicit any reduction in cellular volume, while the response to carbachol was indistinguishable from that seen in crypts from age-matched control animals. These results are consistent with murine CFTR being a cAMP-activated chloride channel inhibited by glibenclamide and resistant to DIDS. A separate chloride conductance activated by calcium mobilization in small-intestinal crypts appears to be independent of CFTR.
    Materialart: Digitale Medien
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  • 9
    ISSN: 1432-2013
    Schlagwort(e): Small-intestinal crypts ; Intestinal secretion ; Cl− conductance ; K+ conductance ; Cell volume ; Patch clamp ; Guinea-pig
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract We have used the perforated-patch whole-cell recording mode of the patch-clamp technique to monitor membrane potential and measured cell volume changes by image analysis, to determine the nature of the response to secretagogues of isolated whole guinea-pig small-intestinal crypts. Vasoactive intestinal polypeptide (VIP) produced a dose-dependent depolarisation (EC50=30 nM) and an increase in membrane conductance that could be potentiated by carbachol. Similar depolarisations were observed with forskolin. The depolarisation induced by 100 nM VIP was smaller when pipette [Cl−] was 60 mM than when it was 145 mM, suggesting an effect through Cl− conductance activation. Carbachol alone produced a hyperpolarisation (EC50=2 μM). The Cl− channel blocker 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB) produced a small hyperpolarization. When VIP was added in the presence of NPPB, the depolarising effect of the peptide was abolished and a hyperpolarisation was observed instead, consistent with the parallel activation of a K+ conductance. Both carbachol (100 μM) and VIP (100 nM) induced a 25%–30% shrinkage of crypts, which was maximal 8 min after addition of the secretagogue. The induced shrinkage was sustained in the continued presence of agonist and was reversed upon washout. Shrinkage induced by the agonists was abolished by increasing extracellular K+ from 6 mM to 20 mM and was inhibited partially in the presence of 100 μM anthracene-9-carboxylic acid in the bath. The decrease in volume induced by 100 nM VIP was totally abolished in the presence of 100 μM NPPB. The results are consistent with the view that both VIP and carbachol induce secretion in small-intestinal crypts. This would lead to a net loss of K+ and Cl− ions from the cytoplasm with a concomitant decrease in cell volume.
    Materialart: Digitale Medien
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  • 10
    ISSN: 1432-2013
    Schlagwort(e): Small-intestinal crypts ; Regulatory volume increase ; VIP-induced volume decrease ; Na+-K+-2Cl− cotransport ; Cell volume ; Guinea pig
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The volume of intact crypts isolated from guinea-pig small intestine has been measured to assess the capacity of the cells to regulate their volume after hypertonic shock or vasoactive-intestinal-peptide (VIP)-induced shrinkage. Crypts exposed to anisotonic medium initially behave as perfect osmometers. Continued exposure to a hypertonic (400 mosmol/l) medium was followed by regulatory volume increase (RVI), which led to complete volume recovery in about 20 min. VIP produced a volume reduction, attributed to KCl loss through channels activated by the secretagogue, without any recovery during exposure to the polypeptide. Removal of VIP led to an increase of cellular volume towards control levels. This volume recovery after secretagogue-induced shrinkage is termed SVI. Both RVI and SVI were abolished by removal of Na+ or Cl− from the bathing solution, by addition of the loop diuretic bumetanide (1 μM), but not by addition of ethylisopropylamiloride (10 μM) or amiloride (1 mM). Cell shrinkage was also observed when tonicity was increased by addition of 100 mM NaCl or 200 mM D-mannitol, but RVI was seen only when NaCl was the added osmolyte. The ion dependence, pharmacological sensitivity and thermodynamic considerations of these effects are consistent with the operation of a Na+-K+-2Cl− cotransport mechanism activated by cell shrinkage and the secretagogue action of VIP.
    Materialart: Digitale Medien
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