ISSN:
1438-2199
Keywords:
Amino acids
;
d-Amino-acid oxidase
;
d-Aminoaciduria
;
Physiological role
;
Mutant mice
;
d-Amino acids
;
Urine
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Urine of mutant ddY/DAO− mice lackingd-amino-acid oxidase activity contained more serine and proline than that of normal ddY/DAO+ mice.d-Amino-acid oxidase treatment of urinary amino acids decreased the serine and proline, suggesting that they containedd-isomers. An HPLC analysis confirmed the presence ofd-serine. Urinary serine and proline contents were not decreased when the ddY/DAO− mice were fed a diet which did not contain supplementaryd-methionine or when they were given water containing antibiotics. These results suggest that thed-serine andd-proline do not derive from thed-methionine supplemented in the diet or from intestinal bacteria. In urine of the ddY/DAO− mice, a substance which seemed to bed-methionine sulfoxide and/ord-methionine sulfone was present. It is probably a metabolite of thed-methionine supplemented in the diet. Thed-aminoaciduria in the mutant mice lackingd-amino-acid oxidase activity indicates that this enzyme is involved in the metabolism of thed-amino acids in normal mice.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00806921
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