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Digitale Medien

High performance liquid chromatography of tamoxifen and metabolites in plasma and tissues (1993)

Lim, C. K. ; Chow, Loretta C. L. ; Yuan, Zhi-Xin ; [weitere]

New York, NY : Wiley-Blackwell

Biomedical Chromatography 7 (1993), S. 311-314

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Details

ISSN: 0269-3879

Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Medizin

Notizen: An isocratic reversed-phase high performance liquid chromatographic method for the determination of tamoxifen and its metabolites in plasma and tissues is described. Plasma or tissue homogenate was extracted with methanol/dimethyl sulphoxide (4:1 v/v). The supernatant after centrifugation was separated on a BDS-Hypersil column with methanol/0.5 M ammonium acetate (75:25 v/v) as the mobile phase. The recoveries of tamoxifen added to plasma and liver tissue homogenate by the extraction procedure were 102± 1.6 and 98±2.4% (mean±SD, n = 6), respectively. The solutes were detected at 280 nm with a detection limit of 0.25 μg/mL for tamoxifen.

Zusätzliches Material: 6 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bmc.1130070606

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