ISSN:
1573-7330
Keywords:
mouse
;
embryo
;
culture medium
;
development
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Purpose Our purpose was to find a medium to enhance mouse zygote development and, hopefully, to apply the results to a coculture system and to enhance the ART pregnancy rate. Design The study was designed to compare different media's support of mouse zygote development with/without serum supplement. The outcome measure was the percentage of mouse zygotes/embryos that developed to the expanded blastocyst and hatchout stage. Results (1) Using human tubal fluid (HTF), one-cell zygotes had a 34.6±5.2% (mean±standard deviation) development rate, and two-cell embryos a 86.5±3.2% development rate. (2) Minimum essential medium alpha (MEM) showed the best results (52.2±14.5%) among Ham's F-10 (19.1±6.3%), HTF (26.8±8.2%), NCTC-135 (38.8±12.6%), MEM with nuclei acid (24.6±10.0%), and Dulbecco's modified Eagle medium (28.0±20.2%). (3) With the serum supplement, there was no significant difference among Ham's F-10 (21.5±23.7), HTF (29.3±10.4%), NCTC-135 (36.5±6.2%), and MEM (38.8±17.9%). Conclusion MEM is the best medium among the six media examined. Preliminary study showed that MEM gave a good clinical pregnancy rate (29%).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02215717
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