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  • 1
    ISSN: 1432-0584
    Keywords: MDS ; PCR ; p53 ; Nonradioactive SSCP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary p53 is one of the most frequently mutated genes in human cancers. Since p53 has been implicated in lymphatic and some myeloid leukemias, such as the blastic phase of chronic myelogeneous leukemia, we sought to address the role of p53 gene mutations within exons 4–9 in myelodysplastic syndromes (MDS), a myeloid preleukemic condition. In order to avoid the potential hazard of using radioactive single-strand conformation analysis (SSCP), we used a nonradioactive SSCP method based on the silver stain of small minigels. In cell lines with known point mutations of the p53 gene, aberrant migrating bands were found. Serial dilutions indicated a sensitivity comparable to radioactive methods. Furthermore, a common polymorphism within the 4th exon of the p53 gene was easily detected. However, of 17 primary samples from patients with MDS, none harbored a p53 gene mutation. We conclude that this nonradioactive method can easily be used to screen for p53-gene mutations, and that p53-gene mutations do not play a major role in the pathogenesis of MDS.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 619 (1993), S. 109-116 
    ISSN: 0044-2313
    Keywords: Perthioborates, RbBS3, TlBS3, Tl3B3S10 ; preparation ; crystal structures ; Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: The Perthioborates RbBS3, TIBS3, and Tl3B3S10.RbBS3 (P21/c, a=7.082(2) Å, b=11.863(4) Å, c=5.794(2) Å, β=106.54(2)°) was prepared as colourless, plate-shaped crystals by reaction of stoichiometric amounts of rubidium sulfide, boron, and sulfur at 600°C and subsequent annealing. TlBS3 (P21/c, a=6.874(3) Å, b=11.739(3) Å, c=5.775(2) Å, β=113.08(2)°) which is isotypic with RbBS3 was synthesized from a sample of the composition Tl2S · 2 B2S3. The glassy product which was obtained after 7 h at 850°C was annealed in a two zone furnace for 400 h at 400→350°C. Yellow crystals of TlBS3 formed at the warmer side of the furnace.Tl3B3S10 (P1, a=6.828(2) Å, b=7.713(2) Å, c=13.769(5) Å, α=104.32(2)°, β=94.03(3)°, γ=94.69(2)°) was prepared as yellow plates from stoichiometric amounts of thallium sulfide, boron, and sulfur at 850°C and subsequent annealing.All compounds contain tetrahedrally coordinated boron. The crystal structures consist of polymeric anion chains. In the case of RbBS3 and TlBS3 nonplanar five-membered B2S3 rings are spirocyclically connected via the boron atoms. To obtain the anionic structure of Tl3B3S10 every third B2S3 ring of the polymeric chains of MBS3 is to be substituted by a six-membered B(S2)2B ring.
    Notes: Farblose, plättchenförmige Kristalle von RbBS3 (P21/c, a=7,082(2) Å, b=11,863(4) Å, c=5,794(2) Å, β=106,54(2)°) entstehen aus einem stöchiometrischen Ansatz von Rubidiumsulfid, Bor und Schwefel bei 600°C und anschließendem Tempern. Die zu RbBS3 isotype Phase TlBS3 (P21/c, a=6,874(3) Å, b=11,739(3) Å, c=5,775(2) Å, β=113,08(2)°) erhält man aus dem Ansatz Tl2S · 2 B2S3. Das nach 7 h bei 850°C erhaltene glasige Produkt wird in einem Zweizonenofen 400 h bei 400→350°C ausgelagert. Gelbe Kristalle der Verbindung bilden sich in der wärmeren Ofenzone. Das ebenfalls gelbe Tl3B3S10 (P1, a=6,828(2) Å, b=7,713(2) Å, c=13,769(5) Å, α=104,32(2)°, β=94,03(3)β, γ=94,69(2)°) bildet sich aus stöchiometrischen Mengen von Thalliumsulfid, Bor und Schwefel bei 850°C und anschließendem Tempern.Die ausschließlich tetraedrisch koordiniertes Bor enthaltenden Verbindungen bilden polymere Kettenstrukturen, die sich im Fall des RbBS3 und TlBS3 aus spirocyclisch über die Boratome verknüpften, nichtplanaren Trithiadiborolan-Ringen aufbauen. Ersetzt man in diesen Anionenketten jeden dritten B2S3-Fünfring durch einen B(S2)2B-Sechsring, so erhält man die Anionenstruktur des Tl3B3S10.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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