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  • 1
    Electronic Resource
    Electronic Resource
    Cloning of aMicrobispora bispora cellobiohydrolase gene inEscherichia coli (1992)
    Springer
    Journal of industrial microbiology and biotechnology 10 (1992), S. 103-110 
    Details
    ISSN: 1476-5535
    Keywords: Cellulase ; Thermophilic ; Monoclonal antibody
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Cellobiohydrolase II was purified from aMicrobispora bispora culture filtrate and a monoclonal antibody to it was prepared. Screening aM. bispora genomic library inEscherichia coli with this antibody yielded three equivalent clones. Subcloning resulted in greater expression, and activity could be monitored using 4-methylumbelliferylcellobioside. Southern analysis provided evidence that there is a single gene coding for CBH II. The original 22-kb fragment was reduced to 4 kb and subcloned into pUC118/119 resulting in a doubling of expression CBH II. The gene was expressed via its own promoter. The optimal pH (6.5) and the optimal temperature (60°C) of the cloned enzyme are similar to that of the native CBH II.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01583842
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  • 2
    Electronic Resource
    Electronic Resource
    Cloning of a Microbispora bispora cellobiohydrolase gene in Streptomyces lividans (1993)
    Springer
    Applied microbiology and biotechnology 38 (1993), S. 631-637 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The cellobiohydrolase II (CBHII) of Microbispora bispora, originally cloned in Escherichia coli, was subcloned into Streptomyces lividans using shuttle vectors pSKN 01 and pSKN 02. The enzyme was secreted from Streptomyces, whereas it was intracellular in E. coli. The yields of CBHII produced by S. lividans transformants were 15–20-fold higher than those produced by E. coli transformants. The optimal pH of M. bispora native cellobiohydrolase and the cloned enzyme from S. lividans is 6.5. The thermal and pH stability of CHBII produced in M. bispora, E. coli and S. lividans were compared. Enzyme produced in E. coli was inactivated more rapidly (k = 0.252 min−1 at 90° C; 90% inactivation after 10 min vs. 0.119 min−1 for the others). CBHII was monitored following electrophoretic separation by reaction with a monoclonal antibody. The apparent molecular mass of the protein produced from the S. lividans clone was 93 kDa, the same as that of the native enzyme, but that of the enzyme produced in E. coli was smaller (82 kDa).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00182802
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic and molecular characterization of an Adh-1 null mutant in tomato (1991)
    Springer
    Molecular genetics and genomics 226 (1991), S. 120-128 
    Details
    ISSN: 1617-4623
    Keywords: Lycopersicon esculentum ; Ethyl methanesulphonate (EMS) treatment ; Allyl alcohol selection ; Alcohol dehydrogenase (ADH) ; Adh-1 null mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Treatment of tomato seeds with ethyl methanesulphonate (EMS) followed by allyl alcohol selection of M2 seeds has led to the identification of one plant (B15-1) heterozygous for an alcohol dehydrogenase (Adh) null mutation. Genetic analysis and expression studies indicated that the mutation corresponded to the structural gene of the Adh-1 locus on chromosome 4. Homozygous Adh-1 null mutants lacked ADH-1 activity in both pollen and seeds. Using an antiserum directed against ADH from Arabidopsis thaliana, which crossreacts with ADH-1 and ADH-2 proteins from tomato, no ADH-1 protein was detected in seeds of the null mutant. Northern blot analysis showed that Adh-1 mRNA was synthesized at wild-type levels in immature seeds of the null mutant, but dropped to 25% in mature seeds. Expression of the Adh-2 gene on chromosome 6 was unaffected. The potential use of the Adh-1 null mutant in selecting rare transposon insertion mutations in a cross with “mutable” Adh-1 + tomato lines is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273595
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    Paper (German National Licenses)
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