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  • 1990-1994  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Intramembranaceous ossification analyses by a proton microprobe (1990)
    Springer
    Histochemistry and cell biology 94 (1990), S. 171-177 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The proton induced X-ray emission method in combination with a proton microprobe was applied to study the intramembranaceous ossification. As material sections of mouse embryo skulls from the 17th and 19th day of gestation were used. The morphology of the sample was examined by routine histochemical procedure performed on the sections adjacent to that irradiated by the proton microprobe. The measurements were made in line scan and raster scan mode. The concentrations of P, S, Cl, K, Ca, Fe and Zn were determined at each irradiated point. The average element concentrations were calculated for four parts of each section (bone, cartilage, mesenchymal tissue close to the bone and mesenchymal tissue in other places). The distributions of Ca and P (less markedly than Ca) concentrations almost exclusively correlate with localization of the bone while S, Cl and K concentrations show preference to the cartilage. The amount of inorganic material in flat bones of the 17-day embryo amounts to 14% of the dry mass. The material is characterized by a Ca/P ratio of bout 1.6 In the embryo 2 days older the amount of the inorganic phase is practically the same (15%) while the Ca/P ratio approaches 2. This suggests the presence of the precursor phase in the flat bone calcification. It is possible that octacalcium phosphate (Ca/P ratio equals to 1.72) is formed at the onset of the flat bone mineralization which transforms rapidly (in 2 days) to a more stable mineral (defective hydroxyapatite).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02440184
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Quantitative and localized element analysis in cross-sections of spruce [Picea abies (L.) Karst.] needles with different degrees of damage (1991)
    Springer
    Trees 5 (1991), S. 95-100 
    Details
    ISSN: 1432-2285
    Keywords: Element content ; Element distribution ; Forest decline ; Micro-PIXE ; Picea abies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Proton-induced X-ray microanalysis (micro-PIXE) permits the simultaneous determination of the content and the distribution of elements with atomic numbers higher than Z = 13 in biological samples. This method was used to investigate element content and localization in cross-sections of 6-month-old spruce needles. It was possible to detect the elements silicon, phosphorus, sulphur, chlorine, potassium, calcium, manganese, iron and zinc in semithin (10 μm) sections of the needles. The localization of the cationic elements like potassium, calcium and manganese was determined in the one-dimensional line scan mode and in the two-dimensional raster scan mode. To demonstrate the usefulness of this method for forest decline research, element content and localization were compared in needles from two trees, which differed in their degree of damage. We were able to detect differences in the amount of cations and in their distribution inside the needles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227491
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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