ISSN:
1573-4838
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
,
Technology
Notes:
The objective of this study was (1) to improve the succinate dehydrogenase (SDH) staining procedure of the filter test system, and (2) to study the suitability of hydrolases as markers for cell vitality by means of fluorescein diacetate, instead of SDH. The test materials included zinc phosphate cements, conventional and light-cured glass ionomer cements, a composite resin, and methylmethacrylate monomer. Four series of experiments were performed using L-929 mouse fibroblasts: (1) original method, (2) 24 h incubation time with procedural modifications; (3) use of FDA as marker for cell vitality; and (4) the agar overlay method. The staining intensity of the cells in the first series was insufficient. In the second series filter staining was good and showed distinct zones of damaged cells. In the third series cell staining was very distinct and easier to handle than with SDH. The results obtained in the second, third, and fourth series were in agreement with results from other cell culture tests. To compare results from different series an evaluation system based on the area of damaged cells was introduced. Correlations were high between series 2 and 3 as well as between 3 and 4. Our results indicate that the modifications of the filter test improve the method.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00121153
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