ISSN:
1573-5079
Keywords:
chlorophyll a′
;
phylloquinone
;
Photosystem I
;
high-performance liquid chromatography
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Fractionated Photosystem (PS) I particles consisting of six, five or two core proteins were analyzed by HPLC for chlorophyll (Chl) a′ and phylloquinone (PhQ). Each particle had a Chl a/P700 molar ratio of 50–55 and contained ca. 2 molecules of Chl a′ per P700. Deliberate control of eluent composition led to isolated elution of PhQ and β-carotene in the normal-phase chromatogram. Based on these a simple HPLC procedure has been established to determine the PhQ/P700 molar ratio, which was ca. 2 for the larger two PS I particles and ca. 1 for the smallest particle, in line with previous reports.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00014748
Permalink
