ISSN:
1435-232X
Keywords:
G6PD
;
electrophoresis
;
deficiency
;
new variants
;
kinetics
;
inhibition
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Biochemical characteristics of one non-deficient fast G6PD variant (Gd Singapore) and six different deficient variants (three new, two Mahidol, one each of Indonesian and Mediterranean) were studied among the Malays of Singapore. TheGd Singapore variant had normal enzyme activity (82%) and fast electrophoretic mobilities (140% in TEB buffer, 160% in phosphate and 140% in Tris-HCl buffer systems respectively). This variant is further characterized by normalK m for G6P; utilization of analogues (Gal6P, 2dG6P; dAmNADP), heat stability and pH optimum. The other six deficient G6PD variants had normal electrophoretic mobility in TEB buffer with enzyme activities ranging from 1 to 12% ofGd B+. The biochemical characteristics identity them to be 2 Mahidol, 1 Indonesian and 1 Mediterranean variants and three new deficient variants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01883603
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