ISSN:
1573-5028
Keywords:
glutelin
;
multigene family
;
Oriza sativa
;
seed storage protein
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Three members have been isolated of an additional glutelin gene subfamily, named subfamily B, consisting of about five members per haploid rice genome. Restriction fragment length polymorphism analysis showed major differences between Japonica and Indica lines, indicating the divergence of the subfamily since the split between the two varieties. While corresponding exons of the subfamily B showed 80 to 88% nucleotide sequence homology, those exons were only 60–65% homologous to those of the glutelin A subfamily [15, 19, 24], distinguishing them from the subfamily A. Intron position and derived polypeptide structure, in addition to the nucleotide sequence, confirm the subfamily B members as glutelins. Analysis of RNA from seeds of different stages of development showed that the subfamily B members were expressed at the same time as those of subfamily A, demonstrating coordinated regulation of the two subfamilies.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00037068
Permalink