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  • 1
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: ZnS:Sm, ZnS:Sm,Cl, and ZnS:Cl thin films were grown by metalorganic chemical vapor deposition using the thd-chelate (thd=2,2,6,6-tetramethyl-3,5-heptanedione) of Sm and hydrogen chloride. The electroluminescence spectrum of the ZnS:Sm,Cl showed three satellite peaks appearing in each of the 7 nm ranges around the three dominant peaks that the ZnS:Sm has originally. The ZnS:Sm,Cl also showed photoluminescence attributed to the self-activated centers, but its intensity was less than 10−2 of that of the ZnS:Cl. The effect of the Cl codoping on Sm3+ luminescent properties is discussed on the basis of the Sm-VZn (zinc vacancy)-Cl complex formation.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1084
    Keywords: CT ; Liver cirrhosis ; Pancreas ; Trophic effect ; Pathology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To evaluate the effect of hepatic dysfunction on pancreatic morphology, the antero-posterior width of the pancreas was measured by CT in 50 non-alcoholic cirrhotic patients without pancreatic disease and 221 age-and sex-matched normal controls. In addition, we reviewed the histology of the pancreas from 13 autopsies of the cirrhotics and 13 controls. The mean width of the pancreatic head in the cirrhotics was 2.94 ∓ 0.34 cm (mean ∓ SD), which was significantly larger than that in the controls (2.17 ∓ 0.32 cm) (P 〈 0.001). The width of the pancreatic body in the cirrhotics was also significantly greater than in the controls (1.95 ∓ 0.24 cm versus 1.50 ∓ 0.30 cm) (P 〈 0.001). Histological investigation revealed that lipomatosis and fibrosis increased with age in the controls, while they were seldom observed in the cirrhotics. And enlargement of acinar cells or of the islets of Langerhans was often seen in the cirrhotics. These data suggested that advanced liver dysfunction provoked pancreatic growth, probably through a trophic effect.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology 17 (1991), S. 875-885 
    ISSN: 1573-5028
    Keywords: glutelin ; multigene family ; Oriza sativa ; seed storage protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three members have been isolated of an additional glutelin gene subfamily, named subfamily B, consisting of about five members per haploid rice genome. Restriction fragment length polymorphism analysis showed major differences between Japonica and Indica lines, indicating the divergence of the subfamily since the split between the two varieties. While corresponding exons of the subfamily B showed 80 to 88% nucleotide sequence homology, those exons were only 60–65% homologous to those of the glutelin A subfamily [15, 19, 24], distinguishing them from the subfamily A. Intron position and derived polypeptide structure, in addition to the nucleotide sequence, confirm the subfamily B members as glutelins. Analysis of RNA from seeds of different stages of development showed that the subfamily B members were expressed at the same time as those of subfamily A, demonstrating coordinated regulation of the two subfamilies.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5028
    Keywords: β-glucuronidase gene ; endosperm-specific expression ; 5′ deletions ; glutelin gene ; rice ; transgenic tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The 5′ upstream region of the rice storage protein type II glutelin gene was examined for its regulatory function in transgenic tobacco. Chimeric genes containing 5′ flanking regions of the glutelin gene transcriptionally fused to the β-glucuronidase (GUS) reporter gene were introduced into the tobacco genome by Agrobacterium tumefaciens-mediated gene transfer. The chimeric genes were expressed specifically in developing seeds, as opposed to leaves and stems, of the transgenic tobacco. Histochemical analysis revealed that the GUS activity was restricted to the endosperm tissue. A deletion series of the 5′ flanking region was created from position -1329 to -74 relative to the transcriptional initiation site and similarly examined in transgenic tobacco. Measurement of GUS activity of the seeds from the transgenic plants bearing the chimeric genes indicated that the region between positions -441 and -237 was required for the temporal and endosperm-specific expression of the GUS activity in tobacco. RNA analysis by northern blotting confirmed the importance of the -441 to -237 region. Addition of up to 888 bp to the -441 deletion resulted in little increase in GUS activity, although all constructs expressing the GUS gene showed a similar tissue and temporal regulation pattern.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-4986
    Keywords: B-Ley active glycolipids ; monoclonal antibody
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The antigen structure of a mouse monoclonal antibody, GOM-2, established by immunization with KATO-III human gastric cancer cells, was examined. GOM-2 reactive glycolipids were prepared from KATO-III cells and treated with endoglycoceramidase. Structural studies of ten GOM-2 reactive oligosaccharides by a combination of glycosidase digestions, methylation, and affinity chromatography on anUlex europeus agglutinin I (UEA-I) column revealed that nine of them had a Y-related B-active difucosylated determinant (B-Ley) and one had a B-active determinant. Affinity chromatography of the purified and modified oligosaccharides on an immobilized GOM-2 column demonstrated that GOM-2 has a novel binding specificity; it binds tightly to the biantennary structure carrying the B-Ley determinant at the termini or the branched structure carrying the B-Ley structure at two nonreducing termini.
    Type of Medium: Electronic Resource
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