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Localization of hepatitis C viral RNA and capsid protein in human liver (1993)

Yamada, Gotaro ; Nishimoto, Hiroshi ; Endou, Hisashi ; [et al.]

Springer

Digestive diseases and sciences 38 (1993), S. 882-887

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ISSN: 1573-2568

Keywords: Hepatitis C virus ; type C chronic hepatitis ; hepatitis C virus RNA ; hepatitis C virus capsid protein ; in situ hybridization

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract In the livers of patients whose sera contained antibodies to C100-3 antigen (anti-HCV) and hepatitis C virus (HCV) RNA, the presence of HCV RNA and HCV capsid protein (CP) antigen was demonstrated byin situ hybridization and immunohistochemistry, respectively. It was found that occasional hepatocytes in four of ten livers from patients whose sera were positive for both anti-HCV and HCV RNA hybridized with antisense as well as sense oligonucleotide DNA probes, whereas the probes did not hybridize with livers from patients whose sera were negative for anti-HCV and HCV RNA. Monoclonal antibody against a synthetic oligopeptide with amino acid sequence of HCV CP reacted with occasional hepatocytes in six of 14 livers from patients whose sera contained these HCV markers, but not with livers from patients whose sera were negative for both of them. These results suggest that HCV proliferates within hepatocytes since both antisense and sense probes hybridized with cytoplasm of the hepatocytes and that the virus matures in the cytoplasm as the capsid proteins were also found in the hepatocytes.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01295915

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In situ localization of male germ cell-associated kinase (mak) mRNA in adult mouse testis: Specific expression in germ cells at stages around meiotic cell division (1992)

Koji, Takehiko ; Jinno, Atsushi ; Matsushime, Hitoshi ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Cell Biochemistry and Function 10 (1992), S. 273-279

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ISSN: 0263-6484

Keywords: In situ hybridization ; v-ros tyrosine kinase ; male germ cell-associated kinase ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: Biochemical analysis of the male germ cell-associated kinase (mak) gene, which was isolated recently by using weak cross-hybridization with the v-ros tyrosine kinase gene, revealed that the gene was highly expressed in mammalian testicular germ cells, but not in ovarian cells. In order to identify the cells which express the mak gene in more detail, we localized mak mRNA in frozen sections of mouse testis by non-radioactive in situ hybridization. In this study, we utilized thymine-thymine (T-T) dimerized mak cDNA as a haptenic, non-radioactive probe, and the signal was detected enzyme-immunohistochemically by using an anti-T-T antibody. As a result, mak mRNA was localized intensely in late pachytene (stage X) and diplotene (stage XI) spermatocytes, and faintly in dividing spermatocytes (stage XII) and early round spermatids (stage I-II), suggesting that the gene may play an important role in the phase around meiotic cell division, but not throughout the entire meiosis.

Additional Material: 2 Ill.