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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Inorganic chemistry 33 (1994), S. 5617-5619 
    ISSN: 1520-510X
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5117
    Keywords: Bacillariophyceae ; Centrales ; Skeletonema potamos ; ecology ; river phytoplankton
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The small, chain-forming centric diatom Skeletonema potamos (Weber) Hasle is one of the most important species of phytoplankton in the River Danube in Hungary. In low water periods from May to October, its abundance is high, contributing 10–20% of total biomass (its fresh weight is 3–6 mg l−1). The first data about its occurrence in Hungary, in the southern Danube stretch, were published by Schmidt & Vörös (1981). The presence of S. potamos was noted in Danube samples taken at the end of fifties near Budapest (Kiss, 1986). This species became abundant in the Hungarian stretch of River Danube at the end of sixties simultaneously with eutrophication. The main factors influencing the quantities of S. potamos populations are floods and temperature. The nutrient supply of the river is very high, therefore it cannot be a limiting factor. The effect of floods is a complex one. Increase in current velocity is not insignificant. Transparency influenced by the suspended matter content is important. In low water periods when suspended matter content is low (10–20 mg l−1), 70–90% of water column belongs to the euphotic zone. For this reason, low water periods favour increases in phytoplankton density. On the basis of this study, we conclude that S. potamos is a warm stenothermic species, with high light demand. Changes in length of pervalvar axis, diameter, S\V ratio and cell number in chains is influenced by temperature, duration of daylight and rate of cell division.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Nonlinear dynamics 4 (1993), S. 635-653 
    ISSN: 1573-269X
    Keywords: Multibody dynamics ; nonlinear vibration ; internal resonance ; energy balance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract This paper presents the ground-work of implementing the multibody dynamics codes to analyzing nonlinear coupled oscillators. The recent developments of the multibody dynamics have resulted in several computer codes that can handle large systems of differential and algebraic equations (DAE). However, these codes cannot be used in their current format without appropriate modifications. According to multibody dynamics theory, the differential equations of motion are linear in the acceleration, and the constraints are appended into the equations of motion through Lagrange's multipliers. This formulation should be able to predict the nonlinear phenomena established by the nonlinear vibration theory. This can be achieved only if the constraint algebraic equations are modified to include all the system kinematic nonlinearities. This modification is accomplished by considering secondary nonlinear displacements which are ignored in all current codes. The resulting set of DAE are solved by the Gear stiff integrator. The study also introduced the concept of constrained flexibility and uses an instantaneous energy checking function to improve integration accuracy in the numerical scheme. The general energy balance is a single scalar equation containing all the energy component contributions. The DAE solution is then compared with the solution predicted by the nonlinear vibration theory. It also establishes new foundation for the use of multibody dynamics codes in nonlinear vibration problems. It is found that the simulation CPU time is much longer than the simulation of the original equations of the system.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Acta mechanica 105 (1994), S. 173-187 
    ISSN: 1619-6937
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Summary Based on Tresca's yield condition and its associated flow rule, a semianalytical method is presented for the calculation of thermal stresses due to steady-state thermal loading in an assembled shrink fit. The calculation is evaluated assuming plane stress conditions, linear elastic-perfectly plastic materials, and linearly temperature dependent yield stresses. Depending on the temperature gradient, different combinations of pure elastic and plastic zones arise in the shaft and in the hub.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1040-452X
    Keywords: Rabbit embryos ; Micromanipulation ; Nuclear transfer ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The objective of this research was to study efficiency of embryo development following transfer of blastomeres into the perivitelline space of oocytes. Single blastomeres from 8-, 16-, and 32-cell embryos were obtained following mucin coat and zona pellucida removal by combined treatments with pronase and acidic phosphate-buffered saline (PBS, pH = 2.5). Blastomeres were separated by pipetting with a fire-polished micropipette following incubation in Ca++-free PBS for 15 min at 39°C. This procedure resulted in over 97% blastomere separation. For ease of blastomere insertion, oocytes were placed in droplets of 0.5 M sucrose in PBS (SPBS) during micromanipulation. To functionally enucleate oocytes some were stained with Hoechst 33342 DNA stain and irradiated. A single 8- or 16-cell blastomere was aspirated into an injection pipette (35 μm or 25 μm at the tip, respectively) and inserted into the perivitelline space of an irradiated or non-irradiated oocyte, but not fused with the oocyte. This micromanipulation procedure did not affect development of individual blastomeres into blastocysts or trophectoderm vesicles when compared with cultured control single blastomeres (P 〉 .05). When the inserted blastomere was induced to fuse with an intact non-irradiated oocyte under an electric field, 56-57% were fused and 39-45% of the fused and activated oocytes developed to morulae or blastocysts. When an inserted blastomere (from 8-32-cell embryos) was induced to fuse with a functionally enucleated oocyte treated by Hoechst 33342 staining, followed by washing and UV-light irradiation, 63-66% of them were fused, but only 15-22% developed to the morula or blastocyst stage. This research demonstrated that the use of hypertonic medium treated oocytes greatly improved the ease and success rate of blastomere subzona insertion, but the value of functionally enucleated oocytes as recipient cells for nuclear transfer requires further investigation.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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