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  • 1
    Electronic Resource
    Electronic Resource
    RFLP analysis of the MHC class III region defines unique haplotypes for the non-obese diabetic, cataract Shionogi and the non-obese non-diabetic mouse strains (1993)
    Springer
    Diabetologia 36 (1993), S. 727-733 
    Details
    ISSN: 1432-0428
    Keywords: MHC class III region ; non-obese diabetic mouse ; non-obese non-diabetic mouse ; cataract Shionogi mouse ; Type 1 (insulin-dependent) diabetes mellitus ; restriction fragment length polymorphisms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The non-obese diabetic (NOD) mouse strain which spontaneously develops diabetes is a model for human Type 1 (insulin-dependent) diabetes mellitus. At least one of several genes controlling diabetes in the NOD mouse has been mapped to the MHC. Although previous experiments have implicated the MHC class II genes in the development of the disease, the existence of other MHC linked susceptibility genes has not been ruled out. In order to identify these susceptibility genes we have further characterized the MHC haplotype of the NOD mouse and two non-diabetic sister strains, the non-obese non-diabetic (NON) and cataract Shionogi (CTS). We have examined the mouse MHC class III region for the presence of homologous genes to 17 newly isolated human MHC class III region genes (G1, G2, G4, G6, G7a/valyl-tRNA synthetase, HSP70, G8, G9, G10, G12, G13, G14, G15, G16, G17 and G18). We detect unique hybridizing DNA fragments for 16 of the 17 genes in six inbred mouse strains (NOD, NON, CTS, B10, BALB/c and CBA/J) indicating that this part of the H-2 region is similar to the human MHC class III region. Using a panel of restriction enzymes we have defined RFLPs for 6 (G2, G6, HSP70, G12, G16, G18) of the 16 cross-hybridizing probes. The RFLPs demonstrate that NOD, NON and CTS mouse strains each have a distinct MHC haplotype in the MHC class III region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00401143
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  • 2
    Electronic Resource
    Electronic Resource
    CD5 mRNA Expression and Auto-Antibody Production in Early Human B Cells Immortalized by EBV (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 31 (1990), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A number of studies have suggested that lymphocytes producing polyreactive antibodies belong to the CD5+ B-cell subset. In this study we have examined CD5 at the cell surface and mRNA levels in EBV-driven cord blood and fetal liver clones previously characterized in terms of their antibody specificities. We show that EBV-immortalized cells can express surface CD5, and that some of the clones not expressing surface CD5 express it at the mRNA level. The complete absence of CD5 mRNA in some polyreactive clones is consistent with the proposition that the production of auto-antibodies and multispecific antibodies is not restricted to the CD5+ B-cell subset.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1990.tb02768.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A deletion panel of the long arm of the X chromosome: subregional localization of 22 DNA probes (1990)
    Springer
    Human genetics 〈Berlin〉 85 (1990), S. 25-30 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two males and two females with different but overlapping deletions on the proximal long arm of the X chromosomes have been investigated. Their karyotypes, which have been well characterized by high resolution banding techniques, are 46,Y,del(X)(pter→q21.1:: q21.33→qter); 46,Y,del(X) (pter→q21.2::q21.31→qter); 46,X,del(X) (pter→q21.31::q24.3→qter) and 46,X,del (X)(pter→q21.1:). A deletion panel, which makes it possible to subdivide the long arm of the X chromosome into seven subregions, has been established using the genomic DNA from the four families, and applied to the fine subregional localization of the loci for 22 DNA probes. Based on the results obtained, the possible location of the loci in question has been narrowed down considerably, in some cases to an area of only 5% of the previously assigned region; hybridization to Southern blots of a panel with well-characterized chromosome deletions is thus a powerful means of localizing DNA probes, especially with respect to the X probes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276321
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Pulsed field gel electrophoretic analysis of the rat major histocompatibility complex class III region shows extensive inter-species conservation (1994)
    Springer
    Mammalian genome 5 (1994), S. 282-287 
    Details
    ISSN: 1432-1777
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Using pulsed field gel electrophoresis (PFGE), we have examined the rat major histocompatibility complex (MHC) for the presence of a number of new class III region genes recently identified in the human MHC. We find homologous genes to the human G1, G2, G4, G7a, G9, G9a, G10, G13, G15, and G18 genes, but not the G8 gene in the rat genome, and show that these are linked to the rat TNF-α and C4/Slp loci. A long-range restriction map has been constructed on the basis of a PFGE analysis which demonstrates extensive co-linearity in the positions of the homologous sequences in the region between the C4/Slp and TNF loci in the rat MHC when compared with that of the human MHC class III region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00389542
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    Paper (German National Licenses)
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