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  • 1990-1994  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of clinical periodontology 21 (1994), S. 0 
    ISSN: 1600-051X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract The polymorphonuclear neutrophil (PMN) appears lo be an important cell in the protection of the host from pathogenic periodontal microorganisms and, despite some reports to the contrary, it is generally assumed that early-onset forms of periodontal disease including both juvenile and rapidly progressing periodontitis are associated with a defect in PMN chemotactic behaviour. The purpose of the present study was to examine the peripheral PMN chemotactic behaviour, using the under agarose method, in 4 groups, namely healthy periodontium group (n= 7), gingivitis group (n= 8), early-onset periodontitis group (n= 17) and adult periodontitis group (n= 8). PMN from early-onset periodontitis patients showed normal random and chemotactic locomotory behaviour when compared with those of PMN from subjects of the other groups. No statistically significant difference could be found among the 4 studied groups, with regard to spontaneous and oriented migration.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1600-051X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract Previous studies have indicated that oxygen-free radicals may cause damage to the periodontal tissues. This study compared the luminol-dependant chemiluminescence response (after stimulation with either opsonized zymosan or Phorbol myristate acetate (PMA)) of peripheral blood polymorphonuclear leukocytes (PMN) isolated from human subjects with a healthy periodontium (n=7), gingivitis (n=8), adult periodontitis (n=8), or early-onset periodontitis (n=17). These results were also compared with those obtained in a larger reference group which consists of 50 subjects without infection or inflammation, selected on the basis of laboratory investigations. An enhanced response was defined as being 2 standard deviations above the reference group mean; a reduced response was. defined as being 2 standard deviations below this mean. Although PMN from patients with either gingivitis or periodontitis were often functionally activated (when compared to the PMN from the reference group), no significant differences could be found between the 4 groups, with regard to the chemiluminescence response means obtained in a basal state or after stimulation.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 39 (1993), S. 104-109 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The psychrotrophic bacterium Pseudomonas fluorescens is a milk contaminant known to secrete a lipase that is a nuisance for the dairy industry but may have a biotechnological interest. Strain MFO secretes this enzyme upon induction under various conditions. Regardless of the inducer and growth temperature, a single enzyme is produced. However, optimal production occurs when the culture is grown at 17.5° C. Other exported proteins (an extracellular protease and two periplasmic phosphatases) have previously been shown to display exactly the same optimal temperature of production. In contrast, constitutive cell-bound esterase and cytochrome oxidase are produced at a roughly constant rate regardless of the growth temperature. The relevance of these results are discussed in terms of multifunctional regulation and interest for the dairy industry.
    Type of Medium: Electronic Resource
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