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  • 1
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Macromolecules 23 (1990), S. 2524-2533 
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of advanced nursing 18 (1993), S. 0 
    ISSN: 1365-2648
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This paper reports our experience of analysing what may well be one of the largest datasets gathered on nursing practice in the United Kingdom The study produced both quantitative and qualitative data and a method had to be devised both for analysing each form of data and for relating the two An inexpensive relational database was chosen for the purpose, and experience of using it is reported Detailed examples are given We look at the strengths and weaknesses of such a tool, and in general it received a positive evaluation For many nursing research projects, it offers some advantages over a conventional statistical package, especially in the following areas offering ease of use, and hence control of the data, by the domain (nursing) specialist, facilitating the analysis of free-text data, allowing the linking of free-text and structured questionnaire data, permitting the testing of conjectures which arise during analysis, handling varying amounts of data per case, providing non-redundant storage of data, permitting the association of machine-readable codes and human-readable labels, and encouraging an exploratory rather than merely analytical approach
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5036
    Keywords: 15N availability ; bioassay ; immobilization ; nitrification ; organic N ; Pinus contorta
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Although a high proportion of fertilizer N may be immobilized in organic forms in the soil, no studies have examined the long-term availability of residual fertilizer 15N in forestry situations. We investigated this by growing lodgepole pine (Pinus contorta) seedlings in surface (0–10 cm) soil sample eight years after application of 15N-urea, 15NH4NO3 and NH4 15NO3 to lodgepole pine in interior British Columbia. After nine months of growth in the greenhouse, seedlings took up an average of 8.5% of the 15N and 4.6% of the native N per pot. Most of the mineral N in the pots without seedlings was in the form of nitrate, while pots with seedlings had very low levels of mineral N. In contrast to the greenhouse study, there was no significantuptake of 15N by trees in the field study after the first growing season, although half of the soil organic 15N was lost between one and eight years after fertilization. This indicates the need to understand the mechanisms which limit the uptake of mineral N by trees in the field, and the possible mismatch of tree demand and mineral N availability.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 29 (1991), S. 1271-1285 
    ISSN: 0887-6266
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Predictions of the rheological properties of concentrated isotropic solutions of stiff polymers are made that improve on those obtained with the approximate Doi theory. Predictions of the shear-rate and concentration dependence of the viscosity η and the first and second normal stress differences N1 and N2 that avoid the closure approximations of Doi are obtained by using a Galerkin numerical solution for the orientation distribution function. The predictions for η and N1, though they neglect the effects of polydispersity in molecular weight, are shown to be in quantative agreement with recent measurements on solutions of poly-γ-benzyl-L-glutamate (PBLG). The polydispersities of a series of commercial PBLG and PBDG samples are measured by gel permeation chromatography, and found to be broad. An attempt is made to account for the effects of polydispersity on the linear viscoelastic storage and loss moduli for PBLG solutions by using the Marrucci-Grizzuti extension of the Doi theory. The attempt fails. The most probable explanation for the failure is that the rotary diffusive motions of the shorter molecules in the mixture are drastically slowed by the matrix of surrounding longer molecules.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 0749-503X
    Keywords: Hansenula ; haemoglobin ; integration ; continuous culture ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Recombinant human haemoglobin A (rHbA) was produced by a leucine-requiring strain of Hansenula polymorpha which had been transformed with an integration vector containing the Saccharomyces cerevisiae LEU2 gene and cDNAs for the expression of α and β globin each driven by the H. polymorpha MOX promoter. After 40 generations in a chemostat it was found that the integrated vector had become amplified in the host strain. In some cases this led to an increase in LEU2 gene dosage, but a loss of globin expression cassettes. In other cases the globin gene dosage also increased. These changes coincided with an increase in rHbA production in the culture, which was reversed when the dilution rate was increased. Isolates from a chemostat culture producing elevated levels of rHbA were grown in fed-batch fermentations, resulting in higher productivities than when inoculated with the parent strain. The rHbA produced was purified and characterized. Oxygen binding studies and electrospray mass spectrometry showed that the rHbA had been processed and assembled correctly, and behaved as a fully functional co-operative tetramer.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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