ISSN:
1573-6776
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary The synthesis of ethyl (R)-4-chloro-3-hydroxybutanoate through the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate with the NADPH-dependent aldehyde reductase ofSporobolomyces salmonicolor AKU 4429 is described. Under preparative scale reaction conditions with the acetone-fractionated aldehyde reductase, the amount of ethyl-4-chloro-3-hydroxybutanoate reached 33.1 mg/ml (85%ee; molar yield, 74.0%). Furthermore, conversion to ethyl (S)-4-chloro-3-hydroxybutanoate occurred on incubation with washed cells ofTrichosporon cutaneum AKU 4864 as the catalyst.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01030758
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