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There is No Correlation Between Function and Lymphokine Production of HBs-Antigen-Specific Human CD4+-Cloned T Cells (1991)

TSUTSUI, H. ; MIZOGUCHI, Y. ; MORISAWA, S.

Oxford, UK : Blackwell Publishing Ltd

Scandinavian journal of immunology 34 (1991), S. 0

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ISSN: 1365-3083

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: The question whether antigen-specific human CD4+ T cells can be classified on the basis of appropriate and fixed lymphokine production patterns and their corresponding functions still remains lo be elucidated. We generated ten CD4+ T-cell clones specific for HBsAg from HBsAg-positive but HBsAb-negative individuals. Seven of these clones exhibited helper activity for HBsAb response, while the three other clones did not. Both helper- and non-helper-type T-cell clones produced interleukin 4 (IL-4) after antigenic stimulation. By stimulation with phytohaem-agglutinin (PHA) plus phorbol myristate acetate (PMA). three of the seven helper-type clones produced interleukin 2 (lL-2) in addition toiL-4. However, the other four helper-type clones did not produce IL-2 by such stimulation, although they continued the production of IL-4. All non-help)er-type T-cell clones produced a large amount of IL-2. and some of them completely became an IL-2 producer after certain stimulation. These results suggested that both helper- and non-helper-type CD4+ T-cell clones specific for HBsAg might have no strict pattern of lymphokine production as m the TH1 /TH2 dichotomy of murine CD4+ T cells. The data also revealed that lymphokine-producing capacity of individual cloned T cells is changeable depending upon the sort of activation.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1365-3083.1991.tb01566.x

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Immunohistochemical demonstration of nuclear S1 proteins in various cells (1990)

Takahashi, K. P. ; Higashi, Y. ; Uchimoto, T. ; [et al.]

Springer

Histochemistry and cell biology 93 (1990), S. 381-384

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ISSN: 1432-119X

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary S1 proteins are present in the nuclear structures sensitive to DNases and RNase. To examine localization of these proteins, an antibody was raised in a rabbit. Indirect immunofluorescence staining revealed that S1 proteins located in the extranucleolar nuclear regions of quiescent myocardial and cerebellar cells as well as actively duplicating mouse 3T3 fibroblasts. They located in euchromatin regions of thymus lymphocytes, with a characteristic aster-like immunofluorescence pattern, and on the border of condensed chromatin areas by deposition of immunogold particles in ultrathin sections of thymus. Thus, S1 proteins may be in a nuclear function assigned to the border of heterochromatin areas, and other than synthesis of DNA or of ribosomal RNA. Possible involvement of S1 proteins in the extranucleolar RNA synthesis is discussed.