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  • 1
    Electronic Resource
    Electronic Resource
    Heat shock protein 60 (GroEL) from Porphyromonas gingivalis: Molecular cloning and sequence analysis of its gene and purification of the recombinant protein (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 119 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Porphyromonas gingivalis is associated with human periodontal disease. We cloned and sequenced the gene for heat shock protein 60 (GroEL, HSP60) from P. gingivalis FDC381. The identified clone carried a 2.6 kb DNA fragment which contained two open reading frames (ORFs) encoding a 9.6- and a 58.4-kDa protein. The translated amino acid sequence of these ORFs showed a high degree of homology with known sequences for GroES and GroEL from several bacterial species and humans. Escherichia coli carrying this clone expressed a 65-kDa protein which was recognized by anti-Mycobacterium leprae HSP60 monoclonal antibody. We purified the 65-kDa protein by DEAE-sepharose chromatography and hydroxyapatite chromatography. This protein was immunogenic and was recognized by sera from a number of patients with periodontal disease. This immunological reactivity and the existence of molecular mimicry between the P. gingivalis GroEL and other HSP homologs may indicate an important role for this molecule in periodontal lesion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb06879.x
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  • 2
    Electronic Resource
    Electronic Resource
    Heat shock protein 60 (GroEL) from Porphyromonas gingivalis: Molecular cloning and sequence analysis of its gene and purification of the recombinant protein (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 124 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb07271.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Distribution of A and B antigens in organs of blood group AB individuals: observations disclosed by a double immunoenzymatic labeling method (1990)
    Springer
    International journal of legal medicine 103 (1990), S. 557-565 
    Details
    ISSN: 1437-1596
    Keywords: ABH blood group antigens ; immunoenzymatic labeling method ; ABH-Blutgruppenantigene ; Immunenzymatische Markierungsmethode
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Die Lokalisation der A- und B-Antigene in den Organen von Personen der Blutgruppe AB wurde mit einer doppel-immunenzymatischen Methode studiert. In den verschiedenen Epithelzellen dieser Organe konnten beide Antigene nachgewiesen werden. Im Hinblick auf die A- und B-Antigene konnten 4 verschiedene Reaktionsmuster klassifiziert werden: Typ 1: Zellen, welche sich sowohl mit Anti-A als auch mit Anti-B-Seren anfärben ließen; Typ 2: Zellen, welche sich nur mit Anti-A-Serum anfdrben ließen; Typ 3: Zellen, welche sich nur mit Anti-B-Serum anfdrben ließen; Typ 4: Zellen, welche mit beiden Seren negativ reagierten. Die Verteilung jedes dieser epithelialen Zelltypen variierte betrachtlich, auch innerhalb desselben Gewebes und derselben Person. Unsere Ergebnisse legen die Vermutung nahe, daß bei Personen der Blutgruppe AB in der Phase der Konversion zu den A- and B-Antigenen eine Dissoziation stattfindet and daß das Ausmaß zu dieser Dissoziation von Gewebe zu Gewebe and von Zelle zu Zelle variiert.
    Notes: Summary The localization of A and B antigens in the organs of blood group AB individuals has been studied using a double immunoenzymatic labeling method. Both A and B antigens were found in the various epithelial cells of these organs, but the epithelial cells could be classified into the following four types depending on the reaction pattern with anti-A and anti-B sera: type 1: cells that stained positive with both anti-A and anti-B sera; type 2: cells that stained positive with anti-A serum only; type 3: cells that stained positive with anti-B serum only; type 4: cells that were negative with both sera. The distribution of each of these epithelial cell types varied considerably, even in the same tissue and individual. Our results seem to suggest that a dissociation in the conversion to the A and B antigens occurs in the tissue of individuals belonging to blood group AB and that the degree of this dissociation varies from tissue to tissue and from cell to cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00202715
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  • 4
    Electronic Resource
    Electronic Resource
    Distribution of A and B antigens in organs of blood group AB individuals: Observations disclosed by a double immunoenzymatic labeling method (1990)
    Springer
    International journal of legal medicine 103 (1990), S. 557-565 
    Details
    ISSN: 1437-1596
    Keywords: ABH blood group antigens ; immunoenzymatic labeling method ; ABH-Blutgruppenantigene ; Immunenzymatische Markie-rungsmethode
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Die Lokalisation der A-und B-Antigene in den Organen von Personen der Blutgruppe AB wurde mit einer doppel-immunenzymati-schen Methode studiert. In den verschiedenen Epithelzellen dieser Organe konnten beide Antigene nachgewiesen werden. Im Hinblick auf die A-und B-Antigene konnten 4 verschiedene Reaktionsmuster klassifiziert werden: Typ 1: Zellen, welche sich sowohl mit Anti-A als auch mit Anti-B-Seren anfärben ließen; Typ 2: Zellen, welche sich nur mit Anti-A-Serum anfärben lie-ßen; Typ 3: Zellen, welche sich nur mit Anti-B-Serum anfärben ließen; Typ 4: Zellen, welche mit beiden Seren negativ reagierten. Die Verteilung jedes dieser epithelialen Zelltypen variierte beträchtlich, auch innerhalb desselben Gewebes und derselben Person. Unsere Ergebnisse legen die Vermutung nahe, daß bei Personen der Blutgruppe AB in der Phase der Konversion zu den A-und B-Antigenen eine Dissoziation stattfindet und daß das Ausmaß zu dieser Dissoziation von Gewebe zu Gewebe und von Zelle zu Zelle vari-iert.
    Notes: Summary The localization of A and B antigens in the organs of blood group AB individuals has been studied using a double immunoenzymatic labeling method. Both A and B antigens were found in the various epithelial cells of these organs, but the epithelial cells could be classified into the following four types depending on the reaction pattern with anti-A and anti-B sera: type 1: cells that stained positive with both anti-A and anti-B sera; type 2: cells that stained positive with anti-A serum only; type 3: cells that stained positive with anti-B serum only; type 4: cells that were negative with both sera. The distribution of each of these epithelial cell types varied considerably, even in the same tissue and individual. Our results seem to suggest that a dissociation in the conversion to the A and B antigens occurs in the tissue of individuals belonging to blood group AB and that the degree of this dissociation varies from tissue to tissue and from cell to cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01261419
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Transient neutropenia after intravenous injection of vindesine in patients with lung cancer (1994)
    Springer
    European journal of clinical pharmacology 47 (1994), S. 25-32 
    Details
    ISSN: 1432-1041
    Keywords: Vinca alkaloids ; vindesine ; lung cancer ; neutrophil sequestration ; neutrophil deformability ; neutrophil filterability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Abstract We have found transient circulating neutropenia and pulmonary sequestration of neutrophils after the intravenous injection of vindesine, a microtubule disruptor. Experiment 1 Ten patients with lung cancer were given a bolus intravenous injection of 3 mg·m-2 vindesine (Fildesine(r)). In all patients, total leukocyte and neutrophil counts in the venous blood fell to 65 % and 47 % of baseline values respectively within 30 min, and returned to baseline values within 6 h. In contrast, the lymphocyte count was stable. Experiment 2 Male Wistar rats were given saline or 0.08 mg·kg-1 vindesine intravenously and were sacrificed after 30 min. Vindesine produced a 58 % reduction in the neutrophil count in the systemic circulation and a threefold increase in the neutrophil/erythrocyte ratio in the pulmonary microvasculature. Experiment 3 We studied the effects of vindesine in vitro on neutrophils and lymphocytes isolated from the venous blood of healthy volunteers. Vindesine (10-5–10-8 mol·1-1) reduced neutrophil deformability (filterability) and induced neutrophil polarization, with reversibility of both effects after washout. These effects of vindesine were completely inhibited by cytochalasin B, an actin filament disrupter. Vindesine did not stimulate the neutrophil functions of adherence to polystyrene tubes, chemotaxis, or superoxide anion generation. The filterability and morphology of lymphocytes were not altered by vindesine. Thus, we conclude that a bolus injection of vindesine produces pulmonary sequestration of neutrophils, which produces circulatory neutropenia, and that it is primarily mediated by a decrease in neutrophil deformability that occurs without activation of the cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193474
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