ISSN:
1432-1793
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The actions of exogenous arachidonic acid on the performance of the isolated and perfused systemic heart of Octopus vulgaris (collected in the Bay of Naples in 1992), and the potential of this heart for eicosanoid synthesis are described. Arachidonic acid induces positive chronotropic and inotropic effects. The positive inotropic effect is apparent only at the lowest concentration used (10−7 M) and in the paced heart preparations, in which a negative relationship between stroke volume and heart rate has been demonstrated. Using 10−5 M arachidonate, which induces the greatest chronotropic effect, a reduction of inotropism is evident which is due to the above negative relationship between stroke volume and heart rate. These effects are scarcely affected by the cyclooxygenase and lipoxygenase inhibitors, indomethacin (IM) and nordihydroguaiaretic acid (NDGA), respectively. On the coronary system of this heart arachidonic acid displays a potent vasoconstrictory action (ca. 100 percent increase of coronary resistance). This effect is potentiated by IM and reduced by NDGA. 14C-arachidonate is metabolized by the octopus ventricle homogenate into the lipoxygenase products (assayed as hydroxy acids) more actively than into cyclooxygenase products (prostanoids: PGE2, PGD2, PGF2α and 6-keto-PGF1α). On the other hand, the Ca-ionophore A23187 enhances the production of cyclooxygenase metabolites much more than of lipoxygenase metabolites.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00381941
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