Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Optimisation of fast protein separations on non-porous silica-based strong anion exchangers (1993)
    Springer
    Chromatographia 37 (1993), S. 603-607 
    Details
    ISSN: 1612-1112
    Keywords: Anion exchange chromatography ; Protein separation ; Non-porous support ; Polymeric stationary phase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The adsorbed coating technology using various vinylpyrrolidone-vinylimidazole copolymer compositions was carried out on 1.7 μm non-porous monodisperse silica. It was shown that the retention properties and the loading capacity for bovine serum albumin (BSA) increases with the amount of vinylimidazole in the copolymer composition. The retention behavior of various proteins as a function of the salt composition in the eluent has been applied to find the optimal conditions for the synthesis of the anion-exchange stationary phase. The suitability of these supports for the fast separation of biological molecules is demonstrated. The best resolution and the highest speed in protein analysis was obtained by using an anion exchanger of low capacity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02274109
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    A model for polybutadiene coatings on porous silica (1993)
    Springer
    Chromatographia 35 (1993), S. 403-409 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Reversed phase packings ; Polybutadiene coating ; Polymer coatings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Non-wetting viscous liquids such as oligobutadiene prefer “active” sites such as pores during the process of physisorption. Thus, polybutadiene (PBD) coatings on porous silica do not result in a homogeneous polymer film but in an inhomogeneous loading where the bulk polymer is mainly sited in the pores of the silica. An increasing polymer loading leads to increasingly filled pores instead of a thicker polymer film. We cannot exclude the possibility that most of the surface is covered at least with a thin polymer film since the chromatographic behaviour is relatively good for polypeptides, which are highly susceptible to the silanol groups of silica.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02278594
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Packing meterials with surface barriers, controlled distribution and topography of ligands for sample clean-up and analysis of biologically active solutes in HPLC (1991)
    Springer
    Chromatographia 31 (1991), S. 507-511 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Restricted access packings ; Packings with surface barriers ; Sample clean-up of biologically active analytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Various types of, so-called, restricted access packings have been developed for sample clean-up and analysis of biologically active solutes by means of HPLC. The unique feature of these packings is that they prevent the access of matrix components such as protein whilst selectively retaining the drug components and their metabolites. This results from the fact that the particles of the packings possess a surface barrier for large solutes at the external or internal surface area and exhibit a controlled gradient in the chemical surface composition. They are grouped, according to their particle structures, into internal-surface reversed phases (ISRP), shielded hydrophobic phases (SHP), semi-permeable surfaces (SPS), dual zone phases (DZP) and mixed functional phases (MFP). The structural characteristics and the chromatographic properties of these packings are reviewwed, especially with regard to their use in the assaying of drugs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02262398
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Microporous hypercrosslinked polystyrene Styrosorb as a restricted access packing in sample clean-up for high performance liquid chromatography. Part I: Evaluation of restricted access properties (1993)
    Springer
    Chromatographia 36 (1993), S. 351-355 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Hypercrosslinked polystyrene ; Styrosorb ; Restricted access packing ; AmperozideR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Styrosorb is a beaded microporous polystyrene with particle sizes between 2 and 4 μm. In spite of hypercrosslinkage the material was seen to swell in organic solvents. The native material is functionalized with Tris-groups at the outer surface of the particles. The average pore diameter was determined as 1.45±0.3 nm from size exclusion data using polystyrene and polyethylene standards in THF. The reversed phase behavior of the restricted access phase Styrosorb was investigated by injection of two test mixtures. Mixture I contained five aromatic amines, mixture II consisted of AmperozideR and four related compounds. The optimum range of mobile phase composition was assessed so that analytes were separated whilst any proteins present were eluted unretained. Due to the small particle size short columns of 29×4 mm can be used for both sample clean-up and analytical separation of AmperozideR and its metabolite.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02263890
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Separation of polycyclic aromatic hydrocarbons under isocratic conditions by a column switching technique (1994)
    Springer
    Chromatographia 39 (1994), S. 175-179 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Column switching ; Columns at different temperatures ; Polycyclic aromatic hydrocarbons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A simple and efficient method of separating a 20-component PAH-mixture (RSM 1647 standard mixture +benzene, toluene, perylene and coronene) by RP-HPLC is described. Separation was by using two Superspher-100 RP-18 cartridges thermostatted at different temperatures under isocratic conditions with water-acetonitrile eluent. The analysis time with complete resolution of all components can be reduced to 15 min.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02274497
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Adsorptive Trennung von m- und p-Chlortoluol an Zeolithen und Alumophosphaten (1991)
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 63 (1991), S. 730-733 
    Details
    ISSN: 0009-286X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cite.330630713
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...