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  • 1
    Electronic Resource
    Electronic Resource
    Monte Carlo simulation of the transport process in the growth of a-Si:H prepared by cathodic reactive sputtering (1990)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 67 (1990), S. 477-482 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The transport process of sputtered Si atoms from the target to the substrate, crossing an argon-hydrogen plasma, is modeled by using Monte Carlo techniques. The hydrogen and argon partial pressures, the dark zone voltage, and the target-substrate distance are varied in the calculations. The effect of the above-mentioned parameters upon the thermalization and energy distribution of atoms arriving at the substrate is calculated, allowing the determination of the growth conditions that minimize the damage produced on the films by highly energetic atoms, while maintaining a reasonable growth rate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.345228
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  • 2
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    La morale di sant' Alfonso (1993)
    Woodstock, Md., etc : Periodicals Archive Online (PAO)
    Theological Studies. 54:4 (1993:Dec.) 770 
    Details
    ISSN: 0040-5639
    Topics: Theology and Religious Studies
    Notes: SHORTER NOTICES
    URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pao:&rft_dat=xri:pao:article:3258-1993-054-04-000046
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  • 3
    Electronic Resource
    Electronic Resource
    Molecular cloning and expression of the VP1 gene of foot-and-mouth disease virus C1 in E. coli: effect on bacterial cell viability (1991)
    Springer
    Applied microbiology and biotechnology 35 (1991), S. 788-792 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The VP1 gene of foot-and-mouth disease virus (serotype C1) has been cloned in Escherichia coli Clts cells, under the control of the bacteriophage lambda p L promoter. The expressed VP1 protein was complete and non-fused, and its molecular weight was indistinguishable from that of the VP1 obtained from virions. Cells harbouring the recombinant vectors exhibited symptoms of plasmid instability and toxicity and died in a few weeks even when never exposed to inducing conditions. A new plasmid clone in which a segment of the VP1 gene was fused with contiguous genes of the viral genome was very stable. The expressed partial VP1 protein contains the two major immunogenic domains of the virion. This system can be used as a tool to design an immunogenic VP1, and to explore possible synthetic vaccines against foot-and-mouth disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00169896
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  • 4
    Electronic Resource
    Electronic Resource
    Cellular and subcellular distribution of 2,4-dinitrophenyl groups in mouse epidermis and regional lymph nodes after epicutaneous application of 2,4-dinitrofluorobenzene (1991)
    Springer
    Archives of dermatological research 283 (1991), S. 251-257 
    Details
    ISSN: 1432-069X
    Keywords: Langerhans cell ; Contact dermatitis ; Antigen processing ; 2,4-Dinitrofluorobenzene ; Antigen-presenting cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The cellular and subcellular distribution of 2,4-dinitrophenyl (DNP) groups in the epidermis and regional lymph nodes of the mouse was investigated after epicutaneous application of 2,4-dinitrofluorobenzene (DNFB) to sensitized and non-sensitized mice. The peroxidase-antiperoxidase method and the immunogold technique were used to visualize the DNP groups at both light and electron microscopic levels. The highest intensity of immunolabelling was found on tonofilaments of keratinocytes present in the upper layers of the epidermis. On the other hand, in vitro experiments showed that DNFB has the capacity to bind keratin which, together with immunocytochemistry, suggests that this molecule may be one of the skin protein carriers for DNFB. In addition, intense immunostaining for DNP was observed in the Golgi area of some epidermal Langerhans cells. Cells immunoreactive to DNP were also observed in the marginal sinus of cervical lymph nodes 6, 12 and 24 h after challenge. Immunoelectron microscopy revealed immunoreactive DNP groups in phagosomes of Langerhans cells at this site. The present findings support the hypothesis that the hapten DNFB penetrates passively into the cytoplasm of Langerhans cells, concentrates in the Golgi area and, during the migration of Langerhans cells to the lymph nodes, it is probably processed in the lysosomes before its presentation to T lymphocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01106111
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  • 5
    Electronic Resource
    Electronic Resource
    Sequential extraction as a tool to study the foliar uptake of radionuclides (1993)
    Springer
    Journal of radioanalytical and nuclear chemistry 173 (1993), S. 377-385 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract In order to study the foliar uptake of radionuclides deposited from an aerosol released in an accidental situation, lettuce plants were contaminated with an aerosol containing85Sr,134Cs and110mAg isotopes. The methodology chosen to carry out this study is based on a sequential extraction scheme using water, to evaluate the fraction possibly removed by rain or by washing, and an organic solvent, to distinguish between the fraction adhering to the cuticle and the one incorporated in the leaves. Two procedures are compared. Moreover, the different behavior of the radionuclides in their incorporation into the leaves is deduced from the results obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02043040
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  • 6
    Electronic Resource
    Electronic Resource
    Prediction capacity of a sequential extraction scheme (1994)
    Springer
    Journal of radioanalytical and nuclear chemistry 181 (1994), S. 85-95 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The predictions of a sequential extraction scheme with respect to the mobility of some radionuclides (85Sr,134Cs and110mAg) in two Mediterranean sandy and sandy-loam soils, are compared to short-term soil-to-plant transfer factors and soil migration. Total soil-to-plant transfer is higher in sandy soil than in sandy-loam soil, as expected and predicted by the scheme. The relative transfer to plants of134Cs and85Sr follows the scheme predictions about exchangeable radionuclide fraction, radiosilver being less mobile than expected. Migration in soil of radiocesium and radiostrontium is also higher in sandy soil, especially for the latter radionuclide, the relative behavior of these two radionuclides being nearer to the bioavailable radionuclide fraction defined by the scheme. However, the scheme fails in predicting radiosilver migration, which is lower than deduced by the scheme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02037550
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  • 7
    Electronic Resource
    Electronic Resource
    A sequential extraction scheme to ascertain the role of organic matter in radionuclide retention in Mediterranean soils (1993)
    Springer
    Journal of radioanalytical and nuclear chemistry 173 (1993), S. 79-86 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A sequential extraction scheme which indicates the role of organic matter in radionuclide retention was applied to two types of Mediterranean soil (sandy-loam and sandy), with low percentages of organic matter and with different contents of clay mineral, which had been previously contaminated with a radioactive aerosol, containing85Sr,134Cs and110mAg. Different distributions were obtained for the three radionuclides, depending on the type of soil.85Sr was the most available radionuclide for both types of soil, showing significant binding to organic matter in sandy-loam soil.134Cs was the most retained radionuclide: for low-organic-matter soils, its availability seemed to depend on clay mineral content.110mAg associated with available organic sites showed a positive correlation with organic matter content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02102700
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  • 8
    Electronic Resource
    Electronic Resource
    Factors controlling spatial variability in ammonium release within an estuarine bay (Alfacs Bay, Ebro Delta, NW Mediterranean) (1992)
    Springer
    Hydrobiologia 235-236 (1992), S. 519-525 
    Details
    ISSN: 1573-5117
    Keywords: sediment ; ammonium release ; redox conditions ; chlorophyll a derivatives ; bell jar incubations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sediment-water ammonium fluxes, oxygen uptake and sediment characteristics were studied in an estuarine bay influenced by temporal freshwater discharges. Sediment at seven stations representing a gradient imposed by freshwater inputs was sampled for sediment-water ammonium and oxygen fluxes, chlorophyll a derivative pigments, organic content, porosity and elemental composition (Fe, Mn, Si, Al). Oxygen uptake decreased along the gradient and correlated with total chlorophyll a derivatives indicating the close coupling between aerobic metabolism and short-time sedimentation events. Ammonium release showed a discontinuous pattern of decrease along the gradient and only correlated with the Fe:Mn atomic ratio. Correlation between the structural properties of the sediment (Si:Al atomic ratio, porosity and organic content) and ammonium release was also found (excluding data from the station with the highest ammonium flux). The extent of the influence of metabolism and sediment structure on ammonium release is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00026239
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  • 9
    Electronic Resource
    Electronic Resource
    Simultaneous on line monitoring of intracellular β-galactosidase activity and biomass using flow injection analysis inEscherichia coli batch fermentations (1992)
    Springer
    Biotechnology techniques 6 (1992), S. 213-218 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A novel method to monitor on-line intracellular β-galactosidase activity and biomass simultaneously, using flow injection analysis (FIA), has been developed. The automatic ultrasonic cell disruption and FIA analysis allow the processing of 10 samples/hour with a wide and variable linear working range of β-galactosidase activity and biomass and a maximum relative standard deviation (RSD) of 1.5%. The system has been optimized by monitoring biomass and intracellular β-galactosidase activity inEscherichia coli batch fermentation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02439346
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  • 10
    Electronic Resource
    Electronic Resource
    Assay of β-galactosidase activity by Flow Injection Analysis (FIA) (1991)
    Springer
    Biotechnology techniques 5 (1991), S. 389-392 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A novel method to analyze β-galactosidase by Flow Injection Analysis is presented with a linear working range extended to at least 2150 U/mL, being the detection limit 25 U/mL with 55 samples per hour frecuency and a BSD of 0.954% versus 2.4% obtained by manual assay. The method was tested with optimal results with samples from Escherichia coli cultures producing β-galactosidase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00185020
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