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Cloning and sequence analysis of a recA-like gene from Streptomyces venezuelae ISP5230 (1994)

Yao, W. ; Vining, L.C.

Oxford, UK : Blackwell Publishing Ltd

FEMS microbiology letters 118 (1994), S. 0

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ISSN: 1574-6968

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: Abstract When genomic DNA fragments from Streptomyces venezuelae ISP5230 were probed at moderate stringency with recA from Mycobacterium tuberculosis a 2.0-kb SmaI fragment was identified. The fragment was isolated by cloning a BamHI digest of S. venezuelae DNA in pHJL400 and screening the plasmids in Escherichia coli by Southern hybridization using a sib-selection technique. Sequencing the hybridizing region located an open reading frame encoding 377 amino acids. Its deduced amino acid sequence resembled that of recA genes from other bacteria. The cloned S. venezuelae gene conferred partial resistance to ethyl methanesulfonate when expressed in E. coli from the lacZ promoter.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb06802.x

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