ISSN:
1618-2650
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The covalent bonding of l-lactate oxidase (no E.C.) to cross-linked copolymers of chitosan (Chitopearl) using the glutaraldehyde method is described and applied to the determination of l-lactate in serums of diseased and normal animals. The bioreactor packing of the immobilized enzyme is stable for at least 4 months and can be continuously used for the rapid and simple determination of l-lactate. The reduction current of pyruvate, corresponding to the product of enzymatic reaction, is determined by differential pulse polarography. The reproducibility (RSD) for 10 μmol/l l-lactate is 0.78% (n=6) and the detection limit is 0.62 μmol/l (k=2, confidence level 97.72%).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00325750
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