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  • 1
    Electronic Resource
    Electronic Resource
    Degradation behaviors of buried heterostructure InGaAsP/InP distributed feedback lasers grown by liquid-phase epitaxy (1988)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 64 (1988), S. 496-499 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Some degradation modes of 1.3 and 1.55 μm buried heterostructure distributed feedback (DFB) lasers grown by liquid-phase epitaxy are clarified. A degradation mode induced by the inhomogeneous optical-field distribution along the laser cavity is found to exist in DFB lasers. In most DFB lasers, the interface degradation between first growth-step (double heterostructure) layers and second growth-step (burying) layers occurs in the same manner as Fabry–Perot lasers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.341987
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  • 2
    Electronic Resource
    Electronic Resource
    Flexible carbonaceous and graphitized films by pyrolytic chemical-vapor-deposition method from hydrocarbons containing oxygen atoms (1989)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 66 (1989), S. 881-884 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Carbonaceous films with high conductivity have been prepared by a pyrolytic chemical-vapor-deposition method using hydrocarbons containing oxygen atoms, phenols, carboxylic acids, etc. The films obtained are self-supporting and flexible. The electrical conductivities of the films depend on the pyrolyzing temperature but not remarkably on the kind of raw materials. The films are converted to high-quality graphitized films with conductivity of 1.6×104 S/cm by heating under an Ar atmosphere to 2800 °C. The electrical conductivity and thermoelectric power of the films are examined as a function of the heat-treatment temperatures. The excellent property of the films obtained in this study seems to arise from the effect of oxygen atoms in the process of condensed aromatic ring formation by pyrolysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.343514
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  • 3
    Electronic Resource
    Electronic Resource
    Immunochemical Studies of the Location of Luteinizing Hormone-Releasing Hormone Neurons in the Nervus Terminalis of the Mouse (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 519 (1987), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1987.tb36317.x
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  • 4
    Electronic Resource
    Electronic Resource
    Application of immunoblotting to serum protein phenotyping with reference to α2HS-glycoprotein (AHS) typing of bloodstains (1985)
    Springer
    International journal of legal medicine 95 (1985), S. 153-158 
    Details
    ISSN: 1437-1596
    Keywords: Serum groups, immunoblotting ; α2HS-glycoprotein typing ; Bloodstains, immunoblotting ; Serumgruppen, Immunoblottierung ; α2HS-Glykoprotein, Typisierung ; Blutspuren, Immunoblottierung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Seren und Blutextrakte wurden auf Polyacrylamidgelen isoelektrisch fokussiert. Die fokussierten Proteine wurden auf Nitrozellulosemembranen durch Diffusion oder elektrophoretisch transferiert. Durch ein spezifisches Antiserum erfolgte auf den Membranen die AG-AK-Reaktion. Nach Waschen der Membranen wurde der gebundene Antikörper mit einem enzymisch markierten Anti-Kaninchen IgG-Antikörper nachgewiesen. Mit dieser Technik konnten die gruppenspezifische Komponente, das α2HS-Glykoprotein, die Komplementkomponenten C6 und C7, der Gerinnungsfaktor 13B und das Plasminogen sehr empfindlich phänotypisiert werden. Vorzugsweise konnten bis zu 6 Monate alte Blutspuren richtig typisiert werden.
    Notes: Summary Sera and bloodstain extracts were subjected to isoelectric focusing in polyacrylamide gels. The focused proteins were transferred to nitrocellulose membranes by diffusion or electrophoretically, then allowed to react with specific antiserum and, after washing, with peroxidase-labeled anti-rabbit IgG. The immune complexes formed on the membranes were detected with 4-chloro-1-naphthol and hydrogen peroxide. Serum group-specific component, α2HS-glycoprotein, the sixth and the seventh component of complement, factor 13B, and plasminogen could be phenotyped with high sensitivity. Bloodstains as old as 6 months could be correctly typed for α2HS-glycoprotein by the blotting technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00201072
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  • 5
    Electronic Resource
    Electronic Resource
    Simultaneous Phenotyping of Genetic Markers for Paternity Testing (1987)
    Springer
    International journal of legal medicine 99 (1987), S. 135-142 
    Details
    ISSN: 1437-1596
    Keywords: Simultaneous phenotyping, genetic markers ; Isoelectric focusing, electrophoresis ; Immunoblotting, paternity testing ; Phänotypisierung genetischer Marker ; Vaterschaftsbegutachtung, kostensparende Methoden und Dokumentation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Es werden zeit- und kostensparende Methoden für die Vaterschaftsbegutachtung beschrieben. Siebzehn genetische Systeme werden in sechs Gruppen unterteilt. 1. Gruppe: Transferrin (Tf), Faktor B (BF) und Phosphoglucomutase 1 (PGM1); 2. Gruppe: Gc-System (Gc) oder α1-Antitrypsin (PI) und α-2HS-Glycoprotein (HSGA); 3. Gruppe: Komplement-Komponente C6 und C7, Faktor 13B (F13B) und Plasminogen (PLG); 4. Gruppe: Haptoglobin (Hp), C8 α-γ Kette (C81) und Faktor I (IF); 5. Gruppe: saure Erythrozyten-Phosphatase (ACP), Esterase D (ESD), Glutamat-Pyruvat-Transaminase (GPT); 6. Gruppe: 6-Phosphogluconat Dehydrogenase (PGD) und Glyoxalase I (GLO). Jede Gruppe wurde gleichzeitig mittels Elektrophorese oder isoelektrische Fokussierung (IEF) mit Färbung oder Immunoblotting untersucht. Diese Methoden erwiesen sich in der Praxis als zeit- und kostensparend und erleichtern die vorübergehende Aufbewahrung und Dokumentation der Elektrophorese-Bilder.
    Notes: Summary Time- and cost-saving methods for paternity testing are described. Seventeen genetic systems were divided into six groups: (1) transferrin (Tf), factor B (Bf), and phosphoglucomutase 1 (PGM1); (2) group-specific component (Gc) or α1-antitrypsin (PI) and α2HS-glycoprotein (HSGA); (3) complement components C6 and C7, factor 13B (F13B), and plasminogen (PLG); (4) haptoglobin (Hp), C8 α-γ chain (C81), and factor I (IF); (5) red cell acid phosphatase (ACP), esterase D (ESD), and glutamic-pyruvic transaminase (GPT); and (6) 6-phosphogluconate dehydrogenase (PGD) and glyoxalase I (GLO). Each group of systems was typed simultaneously by electrophoresis or isoelectric focusing (IEF) followed by staining or immunoblotting. These methods are very practical because they afford a considerable saving of time, work and expense, and facilitate semipermanent preservation of electrophoretic patterns.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00200633
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  • 6
    Electronic Resource
    Electronic Resource
    Examination of previous caesarean section scars by ultrasound (1988)
    Springer
    Archives of gynecology and obstetrics 243 (1988), S. 221-224 
    Details
    ISSN: 1432-0711
    Keywords: Vaginal delivery ; Caesarean section ; Previous caesarean section scar ; Ultrasonography ; Lower uterine segment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We examined 84 lower segment caesarean section scars by ultrasonography near term. Seventy scars showed good healing with a thickness of the lower uterine segment of more than 3 mm; 14 scars showed poor healing with a thickness of less than 2 mm and loss of continuity. Among 70 patients with good healing, 24 patients delivered vaginally but the remaining 46 patients have had repeat caesarean sections for other obstetric indications. Intraoperative findings in these 46 patients were as follows: Grade I (no thinning of the lower uterine segment), 42; Grade II (thinning and loss of continuity of the lower uterine segment but fetal hair not visible), 4; Grade III (thinning of the lower uterine segment and fetal hair visible), 0. Fourteen patients with poor healing had repeat caesarean sections. Intraoperative findings in these 14 patients were as follows: Grade I, 0; Grade II, 9; Grade III, 5. These results indicate that ultrasound examination detect thinning of the lower uterine segment and may help to determine management.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00932271
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  • 7
    Electronic Resource
    Electronic Resource
    Haptoglobin phenotyping by polyacrylamide gel isoelectric focusing and its application to simultaneous typing of serum proteins (1988)
    Springer
    International journal of legal medicine 101 (1988), S. 37-40 
    Details
    ISSN: 1437-1596
    Keywords: Haptoglobin typing, polyacrylamide gel isoelectric focusing ; Blood groups, HP-phenotyping ; Haptoglobin-Typisierung, Isoelektrofokussierung in Polyacrylamidgel ; Blutgruppen, HP-Typisierung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Es wird eine einfache Methode zur Typisierung von Haptoglobin (HP) mittels Isoelektrofokussierung beschrieben. Das Serum wird mit Clostridium perfringens Neuraminidase (CPN), sodann mit Dithiothreitol (DTT) vorbehandelt und anschließend der Polyacrylamidgel-Isoelektrofokussierung (PAGIF) unterworfen. Die Banden wurden durch Immunoblotting sichtbar gemacht. Die Methode erwies sich als erfolgreich bei der HP-Darstellung von selbst 2 Monate alten Blutspuren. Durch eine leichte Modifizierung ist es möglich, neben HP auch die Komplement-Komponente C81 und Faktor I (IF) darzustellen. Immunoblotting ergab außerdem eine dauerhafte Darstellung der HP-Banden. PAGIF ist daher als forensische Routinemethode zur HP-Typisierung geeignet.
    Notes: Summary A simple isoelectric focusing method for haptoglobin (HP) typing is described. Serum was pretreated first with C. perfringens neuraminidase (CPN) and then with dithiothreitol (DTT). The treated serum was subjected to polyacrylamide gel isoelectric focusing (PAGIF), and the band patterns were detected by immunoblotting. The method could be successfully applied to HP typing of bloodstains as old as 2 months. A slight modification of it enabled HP, complement component C81, and factor I (IF) to be typed simultaneously. The immunoblotting facilitated preservation of HP patterns. Thus, the PAGIF method for HP typing is suitable for routine use in the forensic laboratory.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00205322
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  • 8
    Electronic Resource
    Electronic Resource
    Different instability of nuclear DNA at acid hydrolysis in cancerous and noncancerous cells as revealed by fluorescent staining with acridine orange (1986)
    Springer
    Histochemistry and cell biology 84 (1986), S. 556-560 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ehrlich cancer cells and inflammatory cells in mouse ascitic fluid were hydrolyzed and stained with acridine orange (AO). The AO hydrolysis curves for G1/G2+M phase cancer cells and inflammatory cells were differentially determined using flow cytometry by monitoring the metachromatic red-shifted fluorescence of the fluorochrome bound to the single-stranded DNA produced by acid hydrolysis. By computer fitting of the Bateman function to the hydrolysis curves, the kinetic parameters k 1 (rate constant for the degradation of the produced single-stranded DNA), and y 0 (theoretical value of the single-stranded DNA present initially) were determined. It was found that the k 2 value, which reflects the degree of DNA instability, was much higher for cancer cells in both the G1 and G2+M phases than for inflammatory cells. This finding led us to develop a method for the differential AO staining of cancer cells and non-cancerous cells utilizing the different degree of DNA instability at acid hydrolysis. AO staining after hydrolysis with 2N HCl at 30°C for 8.5 min was found to be the optimal method. In the 60 cases of human malignant epithelial and nonepithelial tumors tested, all of the malignant tumor cells emitted metachromatic red fluorescence, while all of the nonmalignant tumor cells (5 cases of benign tumor) and normal cells emitted orthochromatic green fluorescence when observed with a violet excitation light under a fluorescence microscope. This new technique can be a useful tool for the screening of malignancy in exfoliative cytology and also for basic cancer research.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00482991
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  • 9
    Electronic Resource
    Electronic Resource
    Quantitative detection of DNA damage in the neuronal cells of the cerebellum and cerebrum by the analysis of Feulgen hydrolysis curves (1986)
    Springer
    Histochemistry and cell biology 84 (1986), S. 561-565 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Touch smears of the cerebellum and cerebrum of ageing rats were fixed with methanol, hydrolyzed with 2N HCl at various temperatures and for various periods, and stained with pararosaniline-Schiff reagent. The hydrolysis curves were determined by fluorescence cytophotometry and were computer fitted to the Bateman function to determine the kinetic parameters, the initial yield of apurinic acid or single-stranded DNA (y 0), and the rate constants for depurination or denaturation (k 1) and depolymerization (k 2). The values for k 1 (1/k 1 is correlated with the degree of chromatin condensation) and k 2 (which reflects the degree of DNA instability) steadily increased with age. The values for y 0, which may indicate the degree of DNA denaturation or damage present before acid hydrolysis, also increased with age in both the cerebellum and cerebrum; however, this value was lower in the cerebellum untill 15 weeks, with the situation being reversed after 35 weeks, the cross-over time being at about 25 weeks. The values of lnk 1 and lnk 2 were plotted as the function of the reciprocal of the absolute temperature (T) (Arrhenius plot) for both the cerebellum and cerebrum of 15- and 74-week-old rats, and the activation energies (E) for depurination and depolymerization were calculated from the slopes. In particular, the values of E for k 2 decreased much more quickly with age and were smaller in cerebellum. In conclusion, the degree of DNA damage and DNA instability steadily increases in both the cerebellum and cerebrum of ageing rats, and this process is much faster in the cerebellum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00482992
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  • 10
    Electronic Resource
    Electronic Resource
    Immunohistochemical detection of bromodeoxyuridine in formalin-fixed tissues (1986)
    Springer
    Histochemistry and cell biology 85 (1986), S. 193-195 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Bromodeoxyuridine (BrdUrd) immunohisto-chemistry has recently been introduced for the visualization of DNA-synthesizing nuclei. In order to detect the BrdUrd incorporated into nuclear DNA in formalin-fixed, paraffin-embedded tissues, we tested several different pretreatment procedures including digestion with proteinase and hydrolysis with HCl, prior to immunoperoxidase staining. In order to determine the optimal conditions for detecting nuclear BrdUrd, mice were given BrdUrd and 3H-thymidine simultaneously, and the autoradiographic and immunohistochemical results obtained in BrdUrd-stained sections were compared. It was found that digestion with 0:05% proteinase at 37°C for 20 min and hydrolysis with 1 N HCl at 37°C for 20 min was sufficient to detect BrdUrd immunore-activity in 3H-thymidine-labelled nuclei, the results being virtually unaffected by the orders in which the two pretreatments were performed. Our method extends the range of application for BrdUrd, immunohistochemistry in cell-kinetic studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00494803
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