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  • Electronic Resource  (5)
  • 1985-1989  (5)
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  • Electronic Resource  (5)
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  • 1
    Electronic Resource
    Electronic Resource
    Light and electron microscopical postembedding lectin histochemistry for WGA-binding sites in the renal cortex of the mouse embedded in polyhydroxy aromatic resins LR-White and LR-Gold (1988)
    Springer
    Histochemistry and cell biology 89 (1988), S. 277-282 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This work describes a technique which permits study of the postembedding lectin histochemistry for WGA-binding sites at the light and electron microscopical level on the same resin embedded tissue without removing or etching of the resin. Unfixed kidney pieces or kidney pieces fixed in 4% formaldehyde were embedded in the hydrophilic polyhydroxy aromatic resins LR-Gold and LR-White, following dehydration in up to 70% ethanol, 90% ethanol or 100% ethanol. LR-Gold was cryopolymerised at −25° C using the light sensitive initiator benzil, whereas LR-White was heat-cured at +50° C. The localisation of WGA-binding sites at the light microscopical level was investigated using FITC-labelled WGA. The ultrastructural localisation of WGA-binding sites was performed using 15 nm gold-labelled WGA. The best fluorescence staining results were obtained on fixed or unfixed tissue dehydrated in up to 70% ethanol and embedded in LR-Gold. At the ultrastructural level, the best staining results for WGA-binding sites were seen on tissue sections, dehydrated in up to 90% ethanol prior to embedding in LR-Gold.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493152
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Localization of fucosyl moieties in the mouse renal cortex by lectin histochemistry using the fucose binding lectins LTA and UEA I and by autoradiography using 3H-labelled fucose (1988)
    Springer
    Histochemistry and cell biology 89 (1988), S. 505-508 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The binding patterns of the two fucose binding lectins, Lotus tetragonolobus (LTA) and Ulex europeus I (UEA I) were investigated using fluorescence lectin histochemistry on the unfixed renal cortex of the mouse (NMRI) embedded in LR-Gold. The fluorescence staining results were compared with the autoradiographic localization of the incorporation of radioactive fucose into the renal cortex. For this study the turnover of incorporated 3H-fucose in the renal cortex was investigated 30 min, 2 h and 8 h after application. The localization of the radioactive fucose within the renal cortex corresponded well to the labelling pattern observed for lecting histochemistry using LTA. In contrast, with UEA I, no binding sites for this lectin could be observed. The results of our investigation clearly showed that fucosyl moieties in the renal cortex of the NMRI mouse are recognized by the fucose binding lecting LTA, but not by UEA I and that postembedding fluorescence histochemistry with LTA on the LR-Gold embedded kidney is a suitable technique for the localization of fucosyl moieties at the light microscopical level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00492609
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Methodological dependence in the ultrastructural immunolocalization of laminin in tubular basement membranes of the mouse kidney (1987)
    Springer
    Histochemistry and cell biology 87 (1987), S. 59-64 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural localization of the basement membrane glycoprotein laminin was investigated in basement membranes of proximal tubules of the mouse kidney. The localization of laminin was determined using two different immunoperoxidase and one immunogold preembedding technique and one immunogold postembedding technique on unfixed and formaldehyde fixed tissue. Strong differences in the immunolocalization for laminin were found in the lamina densa of the tubular basement membrane using different techniques. After preembedding immunostaining for laminin using JgG-PO as secondary antibody, a positive reaction for the lamina densa was found in the formaldehyde fixed as well as in the unfixed kidney. After preembedding immunostaining for laminin using Protein-A-PO, staining of the l. densa was seen in the unfixed, but not in the fixed kidney. It was striking that no clear immunoreaction in the l. densa of the tubular basement membrane was seen in either the fixed or unfixed tissue after preembedding immunostaining for laminin using protein A-gold. With a direct postembedding immunogold technique laminin was localized only in the l. fibroreticularis and the l. rara but not in the l. densa of basement membranes of proximal tubules of the unfixed and the fixed kidney.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00518725
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Autoradiographical investigations on the affinity of acetyl-(103Ru)-ruthenocene for the adrenal glands of mice (1985)
    Springer
    European journal of nuclear medicine 10 (1985), S. 56-59 
    Details
    ISSN: 1619-7089
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Whole-body autoradiography was used to investigate the distribution of acetyl-103-ruthenium-(103Ru)-ruthenocene in female mice 2 and 24 h after intravenous application. Two hours after the application of acetyl-(103Ru)-ruthenocene, the nasal mucosa, colon, lung, liver, spleen and especially the adrenal glands were labelled. After 24 h, apart from the absence of labelling of the colon, the labelling pattern did not differ from that obtained 2 h after application. Again, the adrenal glands were particularly strongly labelled. Microautoradiography was performed to investigate the distribution of acetyl-(103Ru)-ruthenocene within the adrenal glands. It was shown that acetyl-(103Ru)-ruthenocene labelling was restricted to the zona reticularis and the inner zona fasciculata of the adrenal cortex. After the stimulation of glucocorticoid synthesis in the adrenal cortex by adrenocorticotropic hormone (ACTH) pretreatment, the labelled area in the zone fasciculata was clearly enlarged. It is concluded that acetyl-(103Ru)-ruthenocene has an affinity for those regions of the adrenal glands where adrogen and glucocorticoid synthesis occur.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261764
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    LR-White and LR-Gold resins for postembedding immunofluorescence staining of laminin in mouse kidney (1988)
    Springer
    Journal of molecular histology 20 (1988), S. 427-432 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This work describes a method for the immunolocalization of laminin on 1μm-thick tissue sections using a postembedding immunofluorescence technique. Embedding of unfixed or formaldehyde-fixed mouse renal cortex in either of the acrylic resins LR-White or LR-Gold permitted reliable postembedding immunofluorescence staining for laminin. LR-White was heat-cured at 50°C whereas LR-Gold was polymerized at −25°C. A stronger immunostaining for laminin was obtained from tissue embedded in polymerized LR-Gold compared with the staining from tissue embedded in LR-White. Prerequisites for adequate postembedding immunostaining are the partial dehydration of the tissue (maximum ethanol concentration, 70%) and pepsin treatment of the tissue sections prior to performing the immunostaining reactions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01002428
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    Paper (German National Licenses)
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