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  • Electronic Resource  (3)
  • 1985-1989  (3)
  • 1
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 73 (1987), S. 575-580 
    ISSN: 1432-2242
    Keywords: Somaclones ; Electrophoresis ; Tissue culture ; Medicago sativa L. ; Cluster analysis ; Discriminant analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plantlets were regenerated from alfalfa callus following passage through a tissue culture medium which contained gibberellic acid. A proportion of these plantlets showed obvious morphological variations. Leaflet, stem and petiole tissue of these plants were extracted to yield a soluble protein homogenate which was characterized by polyacrylamide gel electrophoresis. Over 18 individual protein bands were resolved and visualized by staining with coomassie blue G250. Electrophoretic gels from regenerated plantlets and from the parent plant were scanned spectrophotometrically and analyzed. The relative quantity of each of the proteins resolved from plants was correlated with proteins of other plants via the Pearson's product-moment correlation. Cluster analysis was then performed using these correlation coefficients to judge relatedness among somaclones and the parent plant. Two of 22 somaclones (9.1%) differed significantly from the parent and from the other somaclones judged by quantitative protein pattern variations. Three distinctive lineages through tissue culture produced plantlets. Using a discriminant analysis strategy somaclones could be grouped according to lineage with 80.8% accuracy based upon distinctions between protein electrophoretic patterns. Two of the somaclone lineage groupings showed no overlap with the parental grouping which indicated significant molecular divergence of these plantlets as judged by quantitative protein differences.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 75 (1987), S. 189-193 
    ISSN: 1432-2242
    Keywords: Mitochondria ; Mus musculus ; Respiration ; Heterosis ; Complementation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genetically standardized inbred mice exhibit interstrain differences in hepatic mitochondrial respiration rate, respiratory control, efficiency of oxidative phosphorylation with Krebs cycle substrates, and quantity of protein in the mitochondrial fraction. Mitochondria of females of strains with positive combining ability for postweaning growth mixed in vitro show complementation for respiratory traits. Mitochondria of males when mixed do not exhibit complementation. Mitochondria isolated from female mice respire more rapidly, are more tightly coupled and have higher phosphorylation efficiencies compared to those from male mice across all strains assayed. Direct evaluation of androgen effects on mitochondrial respiratory capacity supported this conclusion.
    Type of Medium: Electronic Resource
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