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  • 1995-1999  (2)
  • 1985-1989  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    A simple method for mass propagation of potato (Solanum tuberosum L.) using a bioreactor without forced aeration (1998)
    Springer
    Plant cell reports 18 (1998), S. 284-287 
    Details
    ISSN: 1432-203X
    Keywords: Key words Bioreactor ; Mass propagation ; Potato ; Microtuber
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have developed a simple system for mass propagation of plant organs using a bioreactor without forced aeration. In this system, explants were cultured in bottles equipped with an air-permeable membrane on the cap and these bottles were slowly rotated on a bottle roller. Microtubers of potato were induced using a two-step culture method. In the first step, potato plantlets were cultured under static conditions. After shoot proliferation, the culture medium was replaced with a medium containing a higher concentration of sucrose and the bottles were rotated at 1 rpm. The number of tubers was clearly increased in this system compared to the culture without rotation. The results indicated that our system can be applied for mass propagation of potato tubers at low cost.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050572
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    5-Hydroxyimino-8-quinolone (1997)
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 53 (1997), S. 1324-1325 
    Details
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0108270197006227
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Fine-granular cationic iron colloid (1985)
    Springer
    Histochemistry and cell biology 82 (1985), S. 307-312 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In order to obtain distinct and reliable information concerning the localization of ionized anionic groups in tissues, fine-granular cationic ferric hydroxide colloid solution (Fe-Cac-f) was newly devised. This can be obtained by boiling a mixture of ferric chloride and ammonium cacodylate solutions. the colloid particles of Fe-Cac-f are about 1.0 nm in size, i.e., one-fifth of the size of ferric cacodylate colloid (Fe-Cac; Seno et al. 1983a). As with Fe-Cac, Fe-Cac-f particles in the pH range of 1.6–7.6 carry a positive electric charge, but the latter show a better permeation of tissues. Using the Prussian blue reaction, Fe-Cac-f gives a distinct deep-blue color and can be used for the detection of anionic groups of acid mucopolysaccharides and proteins by light microscopy. It is also useful for detecting the exact sites of ionized anionic groups in deep tissue areas using electron microscopy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00494058
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    A new method of the immunohistochemical detection of cellular antigens for light and electron microscopy (1989)
    Springer
    Histochemistry and cell biology 91 (1989), S. 449-454 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A new immunohistochemical method for light and electron microscopy of tissue- and cell-specific antigens by using ferric colloid-labeled antibody is presented. The antibodies labeled with the cationic cacodylate ferric colloid are stable and bind specifically to the target antigens to show clearly the site of antigens in tissue sections and on free cells by Prussian blue reaction for light microscopy and by the specific figure of electron opaque ferric colloid particles for electron microscopy. The staining procedure is very simple and it gives clear picture. So the method will be of beneficial for general laboratory use in immuno-histochemical researches.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00492514
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    Paper (German National Licenses)
    Fulltext
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