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  • 1995-1999  (1)
  • 1985-1989  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Effects of immobilization on growth, substrate consumption, β-galactosidase induction, and byproduct formation inEscherichia coli (1989)
    Springer
    Journal of industrial microbiology and biotechnology 4 (1989), S. 239-246 
    Details
    ISSN: 1476-5535
    Keywords: Metabolism ; Acetate ; Alginate ; Carbon balance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Some metabolic properties of both suspended and immobilized aerobically and anaerobically growingEscherichia coli cells were investigated. Metabolic activity was found to be substantially different whenE. coli cells were immobilized in alginate. Cells grown immobilized in alginate, and then released from the gel, synthesized 1.6 (aerobic growth) and 4.9 (anaerobic growth) times as much β-galactosidase per cell in response to induction as did suspended cells. Under both aerobic and anaerobic conditions, the cell yield from glycerol for immobilized cells was half that for suspended cells. At specific growth rates that were not significantly different from those of suspended cells, immobilized cells consumed glycerol at twice the rate of suspended cells. Immobilized cells produced elevated quantities of acetate, pyruvate, and lactate. Interpretation of these findings is discussed in terms of the kinetics of energy metabolism and the regulation of inducible protein synthesis inE. coli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01574081
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A model for growth of Saccharomyces cerevisiae containing a recombinant plasmid in selective media (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 29 (1987), S. 469-475 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A major problem in the use of plasmids as recombinant vectors is the problem of plasmid-free cell generation from plasmid shedding and subsequent growth. A common technique for controlling the population of plasmidfree cells is the use of selective media against these cells using an auxotrophic host and a plasmid that has the ability to produced the essential metabolite. A distributed model describing the growth of Saccharomyces cerevisiae containing a recombinant plasmid in selective media was developed. The model allows for growth and production of a metabolite by the plasmid-carrying strain and growth of the plasmid-free cells on resulting metabolite concentrations. Through a determination of system constants and numerical solution to the equations, experimental batch and continuous culture results for cell concentration transients could be simulated by the model. The results indicated that despite selective pressure, plasmid-free cell growth was significant.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260290410
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of oxygen supply on metabolism of immobilized and suspended Escherichia coli (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 51 (1996), S. 697-702 
    Details
    ISSN: 0006-3592
    Keywords: E. coli ; alginate immobilization ; oxygen transport ; mass transfer ; acetate ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effect of reduced oxygen supply on the production of a recombinant protein (plasmid-encoded β-galactosidase) was investigated in Escherichia coli. A novel modified bubble tank reactor was used to provide a direct comparison between immobilized and suspended cells in identical environments except for the immobilization matrix. Decreased oxygen supply led to increased β-galactosidase synthesis by both immobilized and suspended cells. Immobilized cells produced similar amounts of β-galactosidase as the suspended cells. Lactose consumption and acetate production, on a per cell basis, were significantly higher in immobilized cells, suggesting that immobilized cells utilized fermentative metabolism. However, a transport analysis of the immobilized cell system showed that immobilized cells were not subject to either external or internal mass transfer gradients.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
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