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  • 1
    Electronic Resource
    Electronic Resource
    InGaAsP p-i-n photodiodes for optical communication at the 1.3-μm wavelength (1985)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 58 (1985), S. 4730-4732 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The preparation and properties of Cd-diffused p-n homojunction InGaAsP photodiodes designed specifically for operation at the 1.3-μm wavelength are described. At a reverse bias of 10 V, the dark current of these diodes was as low as 15 pA. The peak responsivity at 1.3-μm wavelength was 0.7 A/W. An impulse response (full width at half maximum) of 60 ps and a 3-dB bandwidth of 5.5 GHz were achieved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.336247
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  • 2
    Electronic Resource
    Electronic Resource
    Tensile tests of ropes of very long aligned multiwall carbon nanotubes (1999)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 74 (1999), S. 3152-3154 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We have directly measured the Young's modulus and tensile strength of multiwall carbon nanotubes by pulling very long (∼2 mm) aligned nanotube ropes with a specially designed stress-strain puller. This puller can apply an axial force to the rope and simultaneously measure the corresponding rope elongation and the change in rope resistance. The average Young's modulus and tensile strength obtained were 0.45±0.23 TPa and 1.72±0.64 GPa, respectively, which are lower than those calculated and measured previously. The factors that affect the mechanical strengths of nanotubes are discussed. © 1999 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.124094
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  • 3
    Electronic Resource
    Electronic Resource
    Application of superlattices to the investigation of resonant defect in GaAs layers (1995)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 66 (1995), S. 2256-2258 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: A resonant electron irradiation-induced deep level SE0 in GaAs layers has been investigated by using 50–50 A(ring) uniformly Si-doped GaAs-Ga0.7Al0.3As superlattices and characterized by deep level transient spectroscopy. The formation of a miniband in superlattices changes the forbidden band gap and allows some resonant defects in bulk materials such as SE0 in GaAs to be detected. In these superlattices, three electron irradiation induced deep levels SE0, SE1, and SE2 located in GaAs layers have been observed. In bulk GaAs only two levels, E1 and E2, corresponding to SE1 and SE2 can be detected, since SE0 is a resonant level in bulk GaAs, situated 60 meV above the GaAs conduction band edge. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.113184
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  • 4
    Electronic Resource
    Electronic Resource
    Monoclonal antibody against a major latex allergen reacts with latex products and ammoniated natural latex (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 50 (1995), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Spina bifida patients have been shown to be a high-risk group for latex allergy. Of the several latex proteins identified from nonammoniated natural latex, a polypeptide with a molecular mass of about 23 kDa was shown to be one of the major allergens reacting with IgE in the sera from such patients. Using hybridoma technology, a monoclonal antibody (mAb) 1E2 against the 23-kDa latex allergen was produced. The presence of the 23-kDa or related allergens in finished latex products was evaluated using 1E2. Of the 16 extracts of finished latex products, 12 reacted with 1E2. The other four products are not used in health care. Since the majority of latex products used in health care are manufactured from ammoniated latex and ammoniation may alter latex proteins, we also investigated the effect of ammoniation on the 23-kDa allergen. Although ammoniation degraded latex proteins, the 23-kDa antigen could still be detected by using mAb 1E2 on immunoblot of ammoniated latex proteins. Furthermore, ammoniation resulted in the appearance of additional proteins in high molecular weight positions not present in nonammoniated natural latex, which retained the 23-kDa epitope. The 23-kDa allergen could also be detected on surgical gloves after extensive washing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1995.tb01197.x
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  • 5
    Electronic Resource
    Electronic Resource
    Eicosapentaenoic acid-induced changes in membrane fluidity and cell adhesion molecules in cultured human keratinocytes (1995)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 133 (1995), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The effects of eicosapentaenoic acid (EPA, 20:5n-3) on the lipid dynamics of cultured epidermal keratinocytes and their expression of cell adhesion molecules were investigated, and were compared with those of arachidonic acid (AA, 20:4n-6). When keratinocytes were treated with 3 μg/ml of EPA or AA for 72 h, these compounds were found to be incorporated into the cells. EPA-induced lipid changes were distinguished by a significant increase in the cellular content of n-3 polyunsaturated fatty acids, whereas AA treatment resulted in an increase in the cellular content of n-6 arachidonic acid. These changes in fatty acid composition were accompanied by an increase in cellular membrane fluidity, which was evaluated by the diffusion coefficient, using the method of fluorescence recovery after photobleaching (FRAP) [from 1–77±0–34 × 10−8cm2/s untreated to 2–23±0–35 × l0−8cm2/s EPA-treated (P 〈 0.001), and 2–16±0–35 × 10−8cm2/s AA-treated (P 〈 0·001)]. Intercellular adhesion molecule-1 (ICAM-1) was induced on the keratinocyte membrane in the presence of tumour necrosis factor-a and interferon-γ, and pretreatment with EPA or AA further enhanced the expression, almost to an equal degree, as estimated by flow cytometry (P 〈 0.05). These results indicate that the modulation of ICAM-1 expression does not seem to be EPA-specific, but is presumably a consequence of increased membrane fluidity due to the increased levels of unsaturated fatty acids of both the n-3 and n-6 series in the membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.1995.tb02618.x
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  • 6
    Electronic Resource
    Electronic Resource
    Mechanical characteristics of fiber-filled photo-polymer used in stereolithography (1999)
    Bingley : Emerald
    Rapid prototyping journal 5 (1999), S. 112-119 
    Details
    ISSN: 1355-2546
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The effects of the addition of short glass fibers into an acrylic-based photo-polymer (De Solite SCR310) used in the laser solidification process have been studied. Comparisons of the mechanical properties between pure-polymer specimens and their fiber-filled counterparts were made by subjecting the parts to tensile tests. It was observed that the fiber-reinforced specimens yielded higher measured values of elastic modulus and ultimate tensile strength. The amount of shrinkage encountered by the reinforced prototypes during post-curing was also found to be less than their non-reinforced counterparts. It was also found that the mechanical properties of the post-cured fiber-reinforced specimens were functions of the layer pitch and laser exposure density used during fabrication. By increasing the laser exposure density and decreasing the layer pitch, the mechanical properties of the post-cured fiber-reinforced prototype can be improved, leading to the realization of end products with higher mechanical strengths and better dimensional accuracy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1108/13552549910278937
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  • 7
    Electronic Resource
    Electronic Resource
    Protection from Cell Death by mcl-1 Is Mediated by Membrane Hyperpolarization Induced By K+ Channel Activation (1999)
    Springer
    The journal of membrane biology 172 (1999), S. 113-120 
    Details
    ISSN: 1432-1424
    Keywords: Key words: Apoptosis — 4-Aminopyridine — High K+ concentration — Gene expression — Membrane potential
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract. Mcl-1, a member of the Bcl-2 family, has been identified as an inhibitor of apoptosis induced by anticancer agents and radiation in myeloblastic leukemia cells. The molecular mechanism underlying this phenomenon, however, is not yet understood. In the present study, we report that hyperpolarization of the membrane potential is required for prevention of mcl-1 mediated cell death in murine myeloblastic FDC-P1 cells. In cells transfected with mcl-1, the membrane potential, measured by the whole-cell patch clamp, was hyperpolarized more than −30 mV compared with control cells. The membrane potential was repolarized by increased extracellular K+ concentration (56 mV per 10-fold change in K+ concentration). Using the cell-attached patch-clamp technique, K+ channel activity was 1.7 times higher in mcl-1 transfected cells (NP o = 22.7 ± 3.3%) than control cells (NP o = 13.2 ± 1.9%). Viabilities of control and mcl-1 transfected cells after treatment with the cytotoxin etoposide (20 μg/ml), were 37.9 ± 3.9% and 78.2 ± 2.0%, respectively. Suppression of K+ channel activity by 4-aminopyridine (4-AP) before etoposide treatment significantly reduced the viability of mcl-1 transfected cells to 49.0 ± 4.6%. These results indicate that as part of the prevention of cell death, mcl-1 causes a hyperpolarization of membrane potential through activation of K+ channel activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002329900589
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  • 8
    Electronic Resource
    Electronic Resource
    Separate Domains for Desensitization of GABA ρ1 and β2 Subunits Expressed in Xenopus Oocytes (1998)
    Springer
    The journal of membrane biology 164 (1998), S. 115-124 
    Details
    ISSN: 1432-1424
    Keywords: Key words: Receptor/channel — Retinal ρ1-subunit — Chimera — Voltage-clamp — Barbiturates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract. Desensitization of ligand-gated receptor channels is an intrinsic feedback mechanism and prevents the receptor/channels from becoming overly activated thereby maintaining biological function of the nervous system. Desensitization also plays an important role in neuronal plasticity. By taking advantage of biophysical and pharmacological diversities of GABA β2 subunits from the brain and ρ1 subunits from the retina, structural determinants that confer agonist-induced desensitization were identified. A synthetic chimeric receptor/channel was created from the β2 and ρ1 subunits for this investigation. The chimera was constructed from the extracellular N-domain of the β2 subunit, extending from the amino terminus to the beginning region of the M1 transmembrane segment, and from the C-domain of the ρ1 subunit extending from the M1 transmembrane segment to the carboxyl terminus. The C-domain region included the M1 to M4 transmembrane regions and the large intracellular loop between the M3 and M4 transmembrane segments. Homo-oligomeric GABA β2, ρ1, and β2/ρ1 chimeric receptor/channels were individually expressed in Xenopus oocytes, and the desensitization characteristics attributable to each type of subunit were compared. Results from the present study reveal that motifs in the amino-terminal and carboxyl-terminal domains of the β2 subunit conferred the agonist-induced desensitization; chloroform modulation was linked to specific phases of the GABA-activated current decay.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002329900398
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  • 9
    Electronic Resource
    Electronic Resource
    Effects of Chlorotrifluoroethylene Oligomer Fatty Acids on Recombinant GABA Receptors Expressed in Xenopus Oocytes (1996)
    Springer
    The journal of membrane biology 149 (1996), S. 33-40 
    Details
    ISSN: 1432-1424
    Keywords: Key words: Chlorotrifluoroethylene — GABA receptor — Voltage clamp — Chloride current —Xenopus oocyte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract. GABA-activated Cl− current was expressed in Xenopus oocytes after injecting cRNA that had been transcribed in vitro from complementary DNA (cDNA) coding for a single GABA ρi-subunit cloned from human retina. The expressed current was insensitive to 100 μm bicuculline, but was activated by the GABA analogue trans-4-aminocrontonic acid (TACA). Anion-selective permeability of the expressed ρ1-subunit was determined by isotonically replacing the extracellular Cl− with different anions. The anion permeability was very similar to the native GABAA receptor/channel following a sequence of SCN− 〉 I− 〉 NO3 − 〉 Br−≥ Cl−. Halogenated fatty acids, such as chlorotrifluoroethylene (CTFE) and perfluorinated oligomer acids inhibited the GABA-induced current in oocytes expressing the human retinal GABA ρ1-subunit or rat brain GABAA receptor α1,β2,γ2 subunits. The inhibitory effect of halogenated fatty acids demonstrated a carbon chain length-dependent manner of: C10 〉 C8 〉 C6 〉 C4. Perfluorinated C8-oligomer acid (PFOA) was less effective at blocking this channel than the C8-CTFE oligomer acid. Radiolabeled GABA binding assay indicated that CTFE oligomer acids do not interfere at the GABA binding site of the receptor. Furthermore, the C8-CTFE oligomer fatty acid did not compete with picrotoxin for binding sites within the pore of the channel. These studies demonstrated that the heterologous expression system is useful for studying the molecular interaction between potential neurotoxic agents and neuroreceptors. Our results provide detailed information that should contribute to our understanding of the structure and function of retinal GABA receptors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002329900004
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  • 10
    Electronic Resource
    Electronic Resource
    Preparation of Al-Based Composite Using Mechanical Alloying (1995)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 104-107 (July 1995), p. 111-124 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/43/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.104-107.111.pdf
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