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  • 1
    Electronic Resource
    Electronic Resource
    Patch and whole-cell voltage clamp of single mammalian visceral and vascular smooth muscle cells (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 887-894 
    Details
    ISSN: 1420-9071
    Keywords: smooth muscle ; K channels ; patch clamp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Dispersal of the constituent cells of mammalian visceral and vascular smooth muscles has permitted recordings both of membrane currents under whole-cell voltage clamp, and of currents through single ionic channels using the patch-clamp technique. A rectangular depolarizing step applied to a single cell under voltage clamp yielded a net inward current followed by a net outward current in normal physiological solution. In isolated, ‘inside-out’ patches of cell membrane a calcium- and potential-sensitive K channel (100 pS conductance) and a calcium-insensitive, potential-sensitive K+ channel (50 pS conductance) with slow kinetics have so far been identified and characterized.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01970006
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  • 2
    Electronic Resource
    Electronic Resource
    Voltage-Dependent Calcium Channel in Intestinal and Vascular Smooth Muscle Cells (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 522 (1988), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1988.tb33340.x
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  • 3
    Electronic Resource
    Electronic Resource
    Pregnancy-Associated Plasma Protein-A (PAPP-A) in Preovulatory, Nonovulatory Healthy, and Atretic Human Ovarian Follicles during the Natural Cycle (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 442 (1985), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1985.tb37521.x
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  • 4
    Electronic Resource
    Electronic Resource
    Acetylcholine activates an inward current in single mammalian smooth muscle cells (1985)
    [s.l.] : Nature Publishing Group
    Nature 316 (1985), S. 345-347 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Membrane current and voltage recordings were made from enzymatically dispersed single cells isolated from the longitudinal smooth muscle layer of adult rabbit jejunum7. These cells were 5-15 ??? wide, up to 300 ??? long and contracted on application of acetylcholine. Acetylcholine was applied ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/316345a0
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  • 5
    Electronic Resource
    Electronic Resource
    A simple method of fast extracellular solution exchange for the study of whole-cell or single channel currents using patch-clamp technique (1987)
    Springer
    Pflügers Archiv 410 (1987), S. 335-337 
    Details
    ISSN: 1432-2013
    Keywords: Concentration-jump technique ; Patch-clamp technique ; Single smooth muscle cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A new concentration-jump technique was devised for the rapid application of drugs to single, isolated cells attached to the base of the experimental chamber while recording from them with patch-clamp technique. Cells were placed in a micro-drop (less than 0.1 μl) in a small inner bath which was separated from an outer bath by a ring of “Sylgard” polymer. Stable whole-cell recordings were made in the micro-drop and rapid solution exchange took place when a much larger volume of test solution from the outer bath was flooded over the Sylgard ring and mixed with the micro-drop. Complete equilibration occurred within less than 10 ms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00580285
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  • 6
    Electronic Resource
    Electronic Resource
    Action of nifedipine of BAY K8644 is dependent on calcium channel state in single smooth muscle cells from rabbit ear artery (1988)
    Springer
    Pflügers Archiv 411 (1988), S. 590-592 
    Details
    ISSN: 1432-2013
    Keywords: concentration-jump technique ; patch-clamp technique ; single smooth muscle cell ; 1,4-dihydropyridines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The actions of nifedipine or BAY K 8644 were studied on barium currents recorded from single, collagenase- and elastase-dispersed, smooth muscle cells from the rabbit ear artery using the whole-cell configuration of the patch-clamp technique. Nifedipine (3μM) caused a reduction in the barium current (IBa) evoked by steps to potentials positive of-10mV. This was characterized by a pronounced ‘initial’ block, an increase in the rate of current decay during the voltage-clamp step, but by no increase in block if pulses were repeated every 600ms. Rapid extracellular application of nifedipine (1μM) during the sustained current component (using a new concentration-jump technique) was found to have no effect on IBa over 4s at +20mV, but after returning to the holding potential (-60mV) for 10s, sustained IBa was subsequently abolished. BAY K 8644 (1μM) increased IBa at all potentials, and on rapid application during the sustained current component markedly potentiated IBa. The results suggest that nifedipine binds with high affinity to the closed, available state of the Ca++ channels but they do not suggest binding to the open or inactivated states. The effect of BAY K 8644 is consistent with high affinity binding to the open or inactivated and to the closed, available states.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00582383
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  • 7
    Electronic Resource
    Electronic Resource
    The mechanism of action of Ba2+ and TEA on single Ca2+-activated K+-channels in arterial and intestinal smooth muscle cell membranes (1985)
    Springer
    Pflügers Archiv 403 (1985), S. 120-127 
    Details
    ISSN: 1432-2013
    Keywords: Smooth muscle ; Ba2+ ; TEA ; Ca2+-activated K+ channels ; Patchclamp ; Channel block
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The interaction of Ba2+ and TEA with Ca2+-activated K+ channels was studied in isolated membrane patches of cells from longitudinal jejunal smooth muscle of rabbit and from guinea-pig small mesenteric artery (100 μm external diameter). Ba2+ applied from the inside of the membrane did not reduce unit current, except at high concentrations, but channels failed to open for long periods (s). This effect became much stronger when the potential gradient was in a direction driving Ba2+ into the channel and was reduced by increasing K+ ion concentration on the outside of the membrane. These results are consistent with Ba2+ entering the open channel and blocking at a site most of the way through the channel bore. In contrast, TEA and procaine dose-dependently reduced unit current amplitude at all patch potentials and slightly increased mean open time. Their effects were not detectably voltage-dependent and could be explained by TEA and procaine blocking the open channel with a timecourse that was faster than the frequency response of the recording system. The lack of appreciable voltage-dependence suggests that TEA and procaine bind to a site near to the inner mouth of the channel.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00584088
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  • 8
    Electronic Resource
    Electronic Resource
    The effects of varying the concentrations of ions in the external solution on the oscillations of the membrane potential (Slow Waves) produced by carbachol in longitudinal ileal muscle (1972)
    Springer
    Pflügers Archiv 335 (1972), S. 85-96 
    Details
    ISSN: 1432-2013
    Keywords: Smooth Muscle ; Slow Waves ; Carbachol ; Role of Ions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The membrane potential of the cells of the longitudinal muscle of the guinea-pig ileum was recorded intracellularly with glass microelectrodes. Upon changing from isotonic physiological salt solution to sucrose hypertonic solution the spontaneous electrical activity of the membrane was abolished. Spike discharge, but not slow potential changes, was evoked by depolarizing current. In isotonic or in sucrose hypertonic solution, carbachol or acetylcholine caused spike discharge and produced oscillations of the membrane potential (slow waves) which, in hypertonic solution, were about 20 mV in size and 3 sec in duration. The effects on the response to carbachol of varying the ionic composition were examined in sucrose-hypertonic solution. Slow waves in response to carbachol were rapidly and reversibly abolished in sodium-deficient solution, though electrical stimulation evoked spikes for considerable periods. Slow waves were abolished also in sodium-free solution. In contrast, carbachol evoked slow waves after 20 min in calcium-free solution (in which the membrane depolarized) if the membrane was electrically repolarized. In chloride-deficient solution a small but significant (p〈0.05) increase occurred in the duration of slow waves evoked by carbachol. Carbachol elicited slow waves in potassium-free or in potassium-rich solution. The increases in slow wave size and duration in potassium-free solution fell short of statistical significance (0.1〉p〉0.05). The depolarization produced by carbachol was significantly (p〈0.05) less in sodium-deficient (15 mM) solution but was unaffected by alterations in the external chloride concentrations. In sodium-free solution, carbachol hyperpolarized the membrane. The results support a previous suggestion that the slow waves produced by acetylcholine or carbachol represent an inward sodium current through a slow regenerative ion channel.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00592036
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