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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 51 (1988), S. 0 
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Calf brain 3′-phosphoadenosine 5′-phosphosul-fate (PAPS):proteoheparan sulfate (PHS) N-sulfotransfer-ase activity is solubilized by extracting salt-washed microsomes with 1% Cutscum. A protocol is described for the partial purification of the sulfotransferase activity utilizing: (1) diethylaminoethyl (DEAE)-Sephacel, (2) heparin-Sepharose CL-6B, and (3) 3′,5′-ADP-agarose as chromato-graphic supports. Sulfotransferase activity was followed by using 3′-phosphoadenosine 5′-phospho[35S]sulfate and endogenous acceptors in heat-inactivated microsomes as exogenous substrates. Two chromatographically distinct fractions (ST1 and ST2) of sulfotransferase activity are resolved on DEAE-Sephacel. Both sulfotransferase activities have been partially purified and characterized. An apparent purification of the two N-sulfotransferase fractions of 22– to 29-fold, relative to the microsomal activity, is achieved by this procedure. Since ST1 appears to represent approximately 24% of the total microsomal activity, a purification of 89-fold has been estimated for this fraction. Neither sulfotransferase activity was stimulated by MnCl2, MgCl2, or CaCl2 added at 10 mM, nor inhibited by the presence of 10 mM EDTA. ST1 and ST2 are optimally active at pH 7.5–8. Apparent Km values for PAPS of 2.3 μM and 0.9 μM have been determined for ST1 and ST2, respectively. ST1 exhibits N-sulfotransferase activity primarily and is inhibited by phosphatidylserine whereas the ST2 fraction contains a mixture of N- and O-sulfotransferase activity and is stimulated by phosphatidylserine, phosphatidylcholine, and ly-sophosphatidylcholine. The detection of two chromatographically distinct sulfotransferase activities raises the possibility that N-sulfation of proteoheparan sulfates could be catalyzed by more than one enzyme, and that N-sulfation and O-sulfation of proteoglycans are catalyzed by separate enzymes in nervous tissue.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Psychophysiology 5 (1968), S. 0 
    ISSN: 1469-8986
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine , Psychology
    Notes: SC and SP were measured simultaneously from opposite hands during a stress period and a subsequent prolonged relaxation in 19 Ss. With the Ss relaxed, toward the end of the session, simultaneous measures of SC and SP were taken between various combinations of two active and two drilled reference electrodes on both hands. It was found that SC measured with the external voltage connected in series-adding with the endogenous SP (i.e., positive pole to the active electrode) was some 13 percent higher than when measured in reverse polarity. Supplementary experiment showed that this‘rectification effect’ could be entirely attributed to the effect of the endogenous potential. A method of estimating SC from SP readings with no external current source was shown to give results equivalent to values measured in the usual way. These and other findings support the claim that steady-state electro-dermal properties fit a simple model consisting of a variable voltage and a variable resistance in series. Within-subject correlations of SC and SP were high for Ss with low average SCs, lower for high-SC Ss who seemed less able to relax. The data suggest that SP may be an inverted-U shaped function of arousal and perhaps that the‘beta process,’ which drives the tonic SP downward with increasing arousal, may begin to function at much lower levels of arousal for some Ss than for others. Phasic responses obtained at the end of the session when some Ss were apparently asleep suggest that, when S is drowsy or in light sleep, both the SCR and the SPR have lengthened and variable latencies, and the SPR is uniformly a large, negative going (alpha) response. Uniphasic beta SPRs were rather consistently obtained when the pre-stimulus tonic SP was already high.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Macromolecules 20 (1987), S. 1167-1169 
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of natural products 50 (1987), S. 1186-1186 
    ISSN: 1520-6025
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Industrial & engineering chemistry 57 (1965), S. 54-54 
    ISSN: 1520-5045
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 63 (1988), S. 570-572 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Atomic force microscopy (AFM) is a new technology currently being developed. Stylus profilometry (SP) was developed earlier. A procedure is reported here which combines features of both SP and AFM. In this approach a stylus scans a sample surface and responds to contact forces. Force values are maintained within the elastic range of the sample, and high resolution is achieved by means of a sensitive tunnel gap feedback circuit control. Initial tests of performance have been conducted over a range of applied loads with tungsten styluses on phlogopite mica samples to image lamellar steps with subnanometer resolution.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Reported in this paper are the results of an experiment to produce high-power Ku-band (12–18 GHz) microwave radiation from a backward wave oscillator (BWO) driven by a relativistic electron beam. Experimentally measured output power was about 250 MW at 12.5 GHz and 100 MW at 14 GHz. A description of the slow wave structure is given along with theoretical predictions of the vacuum waveguide dispersion relations. The diagnostics to determine the frequency and power of the device are described. Finally, comparisons between the experimentally measured frequency and power, and the analytic and numerical simulations of the BWO are made.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 66 (1989), S. 3066-3073 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: A self-consistent ensemble Monte Carlo particle model to simulate the dynamics of photogenerated carrier transport in GaAs surface space-charge fields is presented. Dependence of transport parameters, such as relaxation processes, carrier screening, and velocity overshoot, on pulse width, injection level, and excitation energy is investigated and related to the experimentally observable potential drop across the surface space-charge region. In the present study, the high-field conditions are produced by charged surface states, and are to be modified by the redistribution of injected carriers. The photocarrier-induced change in the electric field, which can be measured using electro-optic sampling techniques, is found to be due to the spatial separation of the electron-hole pairs. The simulation results show that at a high injection level a maximum potential drop would result due to complete carrier screening of the surface charge field. The rate of change of this potential drop also would increase with increasing level of injection. These results are in good qualitative agreement with experimental observations. The simulation predicts an enhanced degree of velocity overshoot at decreasing injection energies and, at below L-minima excitation, a second increase in the potential resulting from the redistribution of carriers in the bulk. In this way, it is indicated that the study of the onset of photoconductivity can be used to probe such transport properties in GaAs.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 63 (1988), S. 997-1008 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The electron beam in a linear induction accelerator is generally susceptible to growth of the transverse beam breakup instability. In this paper we analyze a new technique for reducing the transverse coupling between the beam and the accelerating cavities, thereby reducing beam breakup growth. The basic idea is that the most worrisome cavity modes can be cutoff by a short section of coaxial transmission line inserted between the cavity structure and the accelerating gap region. We have used the three-dimensional simulation code SOS to analyze this problem. In brief, we find that the technique works, provided that the lowest TE mode cutoff frequency in the coaxial line is greater than the frequency of the most worrisome TM mode of the accelerating cavity.
    Type of Medium: Electronic Resource
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