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  • 1985-1989  (2)
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  • 1
    Digitale Medien
    Digitale Medien
    Role of protein and calcium in stratum corneum cell cohesion (1987)
    Springer
    Archives of dermatological research 279 (1987), S. 184-189 
    Details
    ISSN: 1432-069X
    Schlagwort(e): Stratum corneum ; Cohesion ; Desquamation ; Protease ; Calcium
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The work reported here indicates that protein and calcium have roles in stratum corneum cell cohesion. A zwitterionic surfactant, 6-octadecyldimethyl ammoniohexanoate (C18AH), was found to completely disaggregate pig and human stratum corneum into intact, individual cells. This method of disaggregation provided a tool to determine the role of tissue components in cell cohesion. The C18AH disaggregation of pig and human stratum corneum was accelerated by proteolytic enzyme and ethylenediaminetetraacetate (EDTA). The C18AH disaggregation could be blocked by pretreatment of the stratum corneum with the serine-type proteolytic enzyme inhibitor phenylmethylsulfonyl fluoride (PMSF). The blockage could be overcome by addition of proteolytic enzyme or divalent metal ion chelator. These and other data indicate the importance of protein and calcium in stratum corneum cell cohesion.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00413255
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Preparation and characterization of a reconstituted stratum corneum film as a model membrane for skin transport (1988)
    Springer
    Archives of dermatological research 280 (1988), S. 252-256 
    Details
    ISSN: 1432-069X
    Schlagwort(e): Stratum corneum ; Skin model ; Skin transport ; Reconstituted skin ; Desquamation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary A zwitterionic surfactant, 6-eicosyldimethyl ammoniohexanoate (C20AH), completely disaggregates stratum corneum into individual cells. The cells can be cast into a film of reconstituted stratum corneum (RSC). Such films prepared from pig and human skin mimic intact human stratum corneum in microscopic, mechanical, and barrier evaluations. The films are useful as model membranes for skin transport experiments.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00513965
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
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