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Digitale Medien

Screening for the presence of the insertion sequence IS1 in the genus Bacteroides (1986)

Larkin, M.J. ; Blakely, G.W. ; Williams, D.R. ; [weitere]

Oxford, UK : Blackwell Publishing Ltd

FEMS microbiology letters 37 (1986), S. 0

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ISSN: 1574-6968

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Biologie

Notizen: Abstract A specific DNA probe, containing a conserved region of the insertion sequence IS1, was hybridised to dot blots of total genomic DNA from 2 oral and 5 intestinal Bacteroides spp. Using Escherichia coli K12 as a positive control and Pseudomonas aeruginosa as a negative control, DNA homologous to the probe could not be detected in Bacteroides corporis, Bacteroides intermedius, Bacteroides ovatus, Bacteroides vulgatus, Bacteroides thetaiotaomicron or 2 strains of Bacteroides fragilis. The total DNA included plasmid DNA of 30.2, 42.7 and 42.7 MDa from B. fragilis, B. intermedius and B. corporis, respectively.IS1 is commonly found in members of the Enterobacteriaceae, and it was concluded that the 2 groups of bacteria are not closely related.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1574-6968.1986.tb01819.x

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