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  • 1
    Electronic Resource
    Electronic Resource
    Interaction of hepatitis B surface antigen with serum albumin of various species on polystylene latex particles (1987)
    Springer
    Medical microbiology and immunology 176 (1987), S. 199-207 
    Details
    ISSN: 1432-1831
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Polystylene latex particles coated with serum albumin of various species, including non-primate serum albumin, were agglutinated by sera containing hepatitis B surface (HBs) antigen and hepatitis Be (HBe) antigen and by purified HBs antigen. Monomer and polymer albumin separated from human serum albumin preparations on latex particles were found to react with HBs antigen. Monomer from non-primate serum albumin preparations bound to latex particles was also found to have the ability to react with HBs antigen, but polymer of non-primate serum albumin did not. The mechanism of reaction between HBs antigen and the latex-bound serum albumin of various species is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00196687
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  • 2
    Electronic Resource
    Electronic Resource
    Flexible carbonaceous and graphitized films by pyrolytic chemical-vapor-deposition method from hydrocarbons containing oxygen atoms (1989)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 66 (1989), S. 881-884 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Carbonaceous films with high conductivity have been prepared by a pyrolytic chemical-vapor-deposition method using hydrocarbons containing oxygen atoms, phenols, carboxylic acids, etc. The films obtained are self-supporting and flexible. The electrical conductivities of the films depend on the pyrolyzing temperature but not remarkably on the kind of raw materials. The films are converted to high-quality graphitized films with conductivity of 1.6×104 S/cm by heating under an Ar atmosphere to 2800 °C. The electrical conductivity and thermoelectric power of the films are examined as a function of the heat-treatment temperatures. The excellent property of the films obtained in this study seems to arise from the effect of oxygen atoms in the process of condensed aromatic ring formation by pyrolysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.343514
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  • 3
    Electronic Resource
    Electronic Resource
    Degradation behaviors of buried heterostructure InGaAsP/InP distributed feedback lasers grown by liquid-phase epitaxy (1988)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 64 (1988), S. 496-499 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Some degradation modes of 1.3 and 1.55 μm buried heterostructure distributed feedback (DFB) lasers grown by liquid-phase epitaxy are clarified. A degradation mode induced by the inhomogeneous optical-field distribution along the laser cavity is found to exist in DFB lasers. In most DFB lasers, the interface degradation between first growth-step (double heterostructure) layers and second growth-step (burying) layers occurs in the same manner as Fabry–Perot lasers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.341987
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  • 4
    Electronic Resource
    Electronic Resource
    Immunochemical Studies of the Location of Luteinizing Hormone-Releasing Hormone Neurons in the Nervus Terminalis of the Mouse (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 519 (1987), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1987.tb36317.x
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  • 5
    Electronic Resource
    Electronic Resource
    Binding characteristics of ionic surfactants with human hair (1986)
    Springer
    Colloid & polymer science 264 (1986), S. 798-802 
    Details
    ISSN: 1435-1536
    Keywords: Binding isotherms ; ionic surfactants ; human hair ; thermodynamic parameReferences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Binding isotherms of two types of ionic surfactants, CmH2m +1SO4Na (m = 8,10,12) and CnH2n + 1N+(CH3)3C1 (n=10, 12), to human hair in aqueous solutions were examined to clarify effects of hydrophobic and electrostatic interaction of ionic surfactants with hair. The binding isotherms of anionic surfactants showed cooperativity with discontinuously increasing shapes, while the binding isotherms of cationic surfactants showed a Langmuir-type, regardless of the difference of a hair condition. The calculated free energy change (— ΔG@#@) for binding, obtained from Klotz' plots, suggests that the binding processes are governed mainly by a hydrophobic interaction, and bound surfactants probably expose their alkyl chains to the aqueous phase, since no-ΔG was observed with the increase of m or n and values of enthalpy change(ΔH) were positive or zero.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01500756
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  • 6
    Electronic Resource
    Electronic Resource
    Quantitative detection of DNA damage in the neuronal cells of the cerebellum and cerebrum by the analysis of Feulgen hydrolysis curves (1986)
    Springer
    Histochemistry and cell biology 84 (1986), S. 561-565 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Touch smears of the cerebellum and cerebrum of ageing rats were fixed with methanol, hydrolyzed with 2N HCl at various temperatures and for various periods, and stained with pararosaniline-Schiff reagent. The hydrolysis curves were determined by fluorescence cytophotometry and were computer fitted to the Bateman function to determine the kinetic parameters, the initial yield of apurinic acid or single-stranded DNA (y 0), and the rate constants for depurination or denaturation (k 1) and depolymerization (k 2). The values for k 1 (1/k 1 is correlated with the degree of chromatin condensation) and k 2 (which reflects the degree of DNA instability) steadily increased with age. The values for y 0, which may indicate the degree of DNA denaturation or damage present before acid hydrolysis, also increased with age in both the cerebellum and cerebrum; however, this value was lower in the cerebellum untill 15 weeks, with the situation being reversed after 35 weeks, the cross-over time being at about 25 weeks. The values of lnk 1 and lnk 2 were plotted as the function of the reciprocal of the absolute temperature (T) (Arrhenius plot) for both the cerebellum and cerebrum of 15- and 74-week-old rats, and the activation energies (E) for depurination and depolymerization were calculated from the slopes. In particular, the values of E for k 2 decreased much more quickly with age and were smaller in cerebellum. In conclusion, the degree of DNA damage and DNA instability steadily increases in both the cerebellum and cerebrum of ageing rats, and this process is much faster in the cerebellum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00482992
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  • 7
    Electronic Resource
    Electronic Resource
    Different instability of nuclear DNA at acid hydrolysis in cancerous and noncancerous cells as revealed by fluorescent staining with acridine orange (1986)
    Springer
    Histochemistry and cell biology 84 (1986), S. 556-560 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ehrlich cancer cells and inflammatory cells in mouse ascitic fluid were hydrolyzed and stained with acridine orange (AO). The AO hydrolysis curves for G1/G2+M phase cancer cells and inflammatory cells were differentially determined using flow cytometry by monitoring the metachromatic red-shifted fluorescence of the fluorochrome bound to the single-stranded DNA produced by acid hydrolysis. By computer fitting of the Bateman function to the hydrolysis curves, the kinetic parameters k 1 (rate constant for the degradation of the produced single-stranded DNA), and y 0 (theoretical value of the single-stranded DNA present initially) were determined. It was found that the k 2 value, which reflects the degree of DNA instability, was much higher for cancer cells in both the G1 and G2+M phases than for inflammatory cells. This finding led us to develop a method for the differential AO staining of cancer cells and non-cancerous cells utilizing the different degree of DNA instability at acid hydrolysis. AO staining after hydrolysis with 2N HCl at 30°C for 8.5 min was found to be the optimal method. In the 60 cases of human malignant epithelial and nonepithelial tumors tested, all of the malignant tumor cells emitted metachromatic red fluorescence, while all of the nonmalignant tumor cells (5 cases of benign tumor) and normal cells emitted orthochromatic green fluorescence when observed with a violet excitation light under a fluorescence microscope. This new technique can be a useful tool for the screening of malignancy in exfoliative cytology and also for basic cancer research.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00482991
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  • 8
    Electronic Resource
    Electronic Resource
    Immunohistochemical detection of bromodeoxyuridine in formalin-fixed tissues (1986)
    Springer
    Histochemistry and cell biology 85 (1986), S. 193-195 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Bromodeoxyuridine (BrdUrd) immunohisto-chemistry has recently been introduced for the visualization of DNA-synthesizing nuclei. In order to detect the BrdUrd incorporated into nuclear DNA in formalin-fixed, paraffin-embedded tissues, we tested several different pretreatment procedures including digestion with proteinase and hydrolysis with HCl, prior to immunoperoxidase staining. In order to determine the optimal conditions for detecting nuclear BrdUrd, mice were given BrdUrd and 3H-thymidine simultaneously, and the autoradiographic and immunohistochemical results obtained in BrdUrd-stained sections were compared. It was found that digestion with 0:05% proteinase at 37°C for 20 min and hydrolysis with 1 N HCl at 37°C for 20 min was sufficient to detect BrdUrd immunore-activity in 3H-thymidine-labelled nuclei, the results being virtually unaffected by the orders in which the two pretreatments were performed. Our method extends the range of application for BrdUrd, immunohistochemistry in cell-kinetic studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00494803
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  • 9
    Electronic Resource
    Electronic Resource
    Examination of previous caesarean section scars by ultrasound (1988)
    Springer
    Archives of gynecology and obstetrics 243 (1988), S. 221-224 
    Details
    ISSN: 1432-0711
    Keywords: Vaginal delivery ; Caesarean section ; Previous caesarean section scar ; Ultrasonography ; Lower uterine segment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We examined 84 lower segment caesarean section scars by ultrasonography near term. Seventy scars showed good healing with a thickness of the lower uterine segment of more than 3 mm; 14 scars showed poor healing with a thickness of less than 2 mm and loss of continuity. Among 70 patients with good healing, 24 patients delivered vaginally but the remaining 46 patients have had repeat caesarean sections for other obstetric indications. Intraoperative findings in these 46 patients were as follows: Grade I (no thinning of the lower uterine segment), 42; Grade II (thinning and loss of continuity of the lower uterine segment but fetal hair not visible), 4; Grade III (thinning of the lower uterine segment and fetal hair visible), 0. Fourteen patients with poor healing had repeat caesarean sections. Intraoperative findings in these 14 patients were as follows: Grade I, 0; Grade II, 9; Grade III, 5. These results indicate that ultrasound examination detect thinning of the lower uterine segment and may help to determine management.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00932271
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  • 10
    Electronic Resource
    Electronic Resource
    Cell proliferation and cell loss in intramucosal signet ring cell carcinoma of canine stomachs induced by N-ethyl-N′-nitro-N-nitrosoguanidine (1985)
    Springer
    Journal of cancer research and clinical oncology 110 (1985), S. 87-94 
    Details
    ISSN: 1432-1335
    Keywords: Stomach ; Signet ring cell carcinoma ; Cell turnover ; Tritiated thymidine autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Signet ring cell carcinoma was induced in canine stomachs by N-ethyl-N′-nitro-N-nitrosoguanidine, and modes of cell proliferation and turnover in the carcinoma were studied by 3H-thymidine autoradiography in conjunction with morphometric analysis. From 2 to 15 months after the cessation of 8 months carcinogen treatment, carcinomas in an early stage were obtained. Most of the cancer tissues confined to the lamina propria showed a layered structure. This comprised three layers; the superficial and the deep layer were composed of signet ring cells, and the middle layer was composed of small round cells. The dogs were labeled with 3H-thymidine by s.c. injection and by local infusion of the celiac artery. Flash-labeled autoradiographs revealed that most 3H-thymidine incorporating cancer cells were located around the middle layer, with a small amount of mucin. Using a pulse labeling experiment, those labeled carcinoma cells were shown to migrate from the middle layer towards the surface. Morphometric analysis of the autoradiographs showed that the small cells in the middle layer migrated upwards and produced mucin to become full-blown signet ring cells by 5.5 days. In 15 days, most labeled cancer cells in the superficial layer had disappeared. This mode of cellular turnover appeared to mimic a cell renewal system of the normal gastric mucosa. If the cancer cells turn over in this way, the tumor must grow slowly, remaining as an intramucosal cancer for a relatively long period.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402717
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