ISSN:
1432-2048
Keywords:
Anthocyanin biosynthesis
;
Dihydroflavonol reductase
;
Flavan-3,4-diol
;
Matthiola
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A cell-free extract from flowers of Matthiola incana catalyzes a NADPH-dependent stereospecific reduction of (+)-dihydrokaempferol to 3,4-cis-leucopelargonidin (5,7,4′-trihydroxyflavan-3,4-cis-diol). The pH-optimum of this reaction is around 6. The rate of reaction with NADH was about 50% of that found with NADPH. (+)-Dihydroquercetin and (+)-dihydromyricetin were also reduced by the enzyme preparation to the corresponding flavan-3,4-cis-diols. Correlation between the genotype of M. incana and the presence of dihydroflavonol 4-reductase is strong evidence that this enzyme is involved in anthocyanin biosynthesis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00395052
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