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  • 1
    Electronic Resource
    Electronic Resource
    Evidence for a direct relationship between mitochondrial genome organization and regeneration ability in hexaploid wheat somatic tissue cultures (1988)
    Springer
    Current genetics 14 (1988), S. 387-394 
    Details
    ISSN: 1432-0983
    Keywords: Triticum aestivum ; Genomic variability ; Mitochondrial DNA ; Somatic tissue culture ; Regeneration ability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Embryogenic and non-embryogenic long-term callus cultures of hexaploid wheat exhibit differences in the organization of their mitochondrial genome. Embryogenic and non-embryogenic fractions of callus cultures initiated from immature embryos of the wheat cultivar “Chinese Spring” have been isolated and subsequently subcultured. DNA-DNA hybridization experiments using labelled cloned wheat mitochondrial DNA fragments have shown that the mitochondrial DNA organization of embryogenic subcultures derived from embryogenic parts of “Chinese Spring” calli is closely related to that of the initial “Chinese Spring” calli, while non-embryogenic subcultures derived from non-embryogenic fragments of “Chinese Spring” calli exhibit a mitochondrial DNA organization similar to that found in non-embryogenic calli derived from cultivar “Aquila”. In addition, somatic tissue cultures initiated from three other non-embryogenic wheat cultivars (“Talent”, “Thésée” and “Capitole”) display mitochondrial DNA arrangements similar to those found in cultivar “Aquila”. These results strongly suggest that, in wheat callus cultures, a particular mitochondrial genome organization is correlated with the ability of cultured cells to regenerate whole plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419997
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  • 2
    Electronic Resource
    Electronic Resource
    Organelle genome stability in anther-derived doubled haploids of wheat (Triticum aestivum L., cv. ‘Moisson’) (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 320-324 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Anther-derived doubled haploids ; Chloroplast DNA ; Mitochondrial DNA ; Restriction patterns
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chloroplast and mitochondrial compartments of a parental line of wheat (Triticum aestivum L., cv. ‘Moisson’) and its anther-derived doubled haploid lines have been analyzed and compared on the basis of their DNA restriction patterns. The results obtained show that no noticeable difference can be detected between doubled haploid lines and parental line at the level of ctDNA and mtDNA organization. It may be concluded that in vitro culture by itself does not systematically generate a cytoplasmic variation in germ cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252074
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  • 3
    Electronic Resource
    Electronic Resource
    Ribosomal DNA as a convenient probe to follow segregation and possible divergency from expected homozygosity after haploidization of an androgenetic process (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 389-396 
    Details
    ISSN: 1432-2242
    Keywords: Ribosomal DNA ; Restriction fragment length polymorphism ; Anther-derived doubled haploids ; Gametoclonal variation ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction fragment length polymorphism of the wheat nuclear ribosomal DNA has been studied in several steps of a breeding scheme, including parental genotypes, F1 hybrid, F9 generation, and anther-derived doubled haploid lines obtained from F9. Ribosomal DNA represents a suitable molecular marker in following segregation and possible divergency from expected homozygosity after haploidization of an androgenetic process. It has been shown to undergo variations among the first cycle-doubled haploid lines in the relative amount of two different sizes of ribosomal DNA repeat units. The specificity and peculiar properties of the plant system used allowed us to assign an intrachromosomal location (short arm of the chromosomes 1B, 1R or 6B) to several ribosomal DNA repeat units that differ by the length of their nontranscribed spacer region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276740
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  • 4
    Electronic Resource
    Electronic Resource
    Gametoclonal variation detected in the nuclear ribosomal DNA from doubled haploid lines of a spring wheat (Triticum aestivum L., cv. ‘César’) (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 31-37 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Anther-derived doubled haploids ; Gametoclonal variation ; Ribosomal DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The organization of the nuclear ribosomal DNA from a parental line of wheat (Triticum aestivum L., cv. ‘César’) and its anther-derived first cycle and second cycle doubled haploid lines has been analyzed by DNA-DNA molecular hybridization. Restricted DNA has been probed by three subclones of wheat nuclear ribosomal DNA covering the entire repeat unit. No significant difference was detected in the extent of methylation of ribosomal DNA of the doubled haploid lines with respect to the parental line. On the other hand, a variation has been found in the organization of the nontranscribed spacer region of ribosomal DNA of the first cycle doubled haploid line. This variation remains stable after a second cycle of in vitro androgenesis. However, one out of five second cycle doubled haploid lines so far tested showed an additional hybridization band present in the parental line but lacking in the first cycle doubled haploid line.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290079
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  • 5
    Electronic Resource
    Electronic Resource
    Identification of new mitochondrial genome organizations in wheat plants regenerated from somatic tissue cultures (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 169-175 
    Details
    ISSN: 1432-2242
    Keywords: Mitochondrial DNA ; Chondriome variability ; In vitro culture ; Plant regeneration ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plants have been regenerated from short-and long-term in vitro somatic tissue cultures made from immature embryos of the hexaploid wheat cultivar “Chinese Spring”. The mitochondrial genome organization of each regenerated plantlet was studied, after one selfing, by probing Sal I-restricted total DNA with cloned Sal I fragments of wheat mitochondrial DNA derived from a segment of the genome, which displays marked structural changes in response to in vitro culture. Short-term in vitro cultures give rise to regenerated plants whose mitochondrial genome organization is either close to that of the parental cultivar or to that of embryogenic callus cultures, except for a single plant which has an organization resembling that of short-term non-embryogenic cultures. In contrast, all but one of the plants regenerated from long-term cultures exhibited a mitochondrial genome organization similar to that of long-term nonembryogenic cultures. In addition, extra labelled bands were detected in some of the regenerated plants with two of the probes used. These results emphasize the importance of the duration of the in vitro step preceding the regeneration process: the longer it is, the higher the probability is of obtaining mitochondrial DNA variability in regenerated plants. Furthermore, since increasing the duration of the in vitro stetp results in the production of regenerated plants with a mitochondrial genome organization resembling that of non-embryogenic tissue cultures, the question is thus raised as to whether regeneration from long-term cultures is suitable for use in plant breeding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266182
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  • 6
    Electronic Resource
    Electronic Resource
    Hirninfarkt nach traumatischer Karotisthrombose (1987)
    Springer
    International journal of legal medicine 99 (1987), S. 219-226 
    Details
    ISSN: 1437-1596
    Keywords: Traumatic carotid thrombosis, cerebral infarction ; Cerebral infarction, traumatic carotid thrombosis ; Traumatische Karotisthrombose, Hirninfarkt ; Hirninfarkt, traumatische Karotisthrombose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Obduktionsfall eines 45jährigen Mannes. Vor 11 Jahren leichtes Schädeltrauma bei Verkehrsunfall, 5 Jahre später Hirninfarkt infolge Karotisthrombose. Histologisch Nachweis einer traumatischen Gefäßwandläsion. Widersprüchliche gutachterliche Stellungnahmen zum fraglichen Kausalzusammenhang zwischen Gefäßverschluß und vorausgegangenem Trauma. In der Berufungsverhandlung Vergleich zwischen der Witwe des Verstorbenen und der Haftpflichtversicherung des Unfallverursachers.
    Notes: Summary An autopsy case of a 45-year-old man is presented. The man had sustained a minor head injury in a traffic accident 11 years ago previously and 5 years later had a cerebral infarction due to thrombosis of the carotid artery. There was histological evidence of a traumatic lesion of the vessel wall. Several contradictory expert opinions were rendered regarding the questionable causal relationship between occlusion of the artery and the previous trauma. In the court of appeal, an agreement was arrived at between the widow of the deceased and the third-party insurance of the person responsible for the accident.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00201254
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Mössbauer spectra of novel gold compounds and their implications for the assignment of molecular structures (1988)
    Springer
    Hyperfine interactions 40 (1988), S. 335-338 
    Details
    ISSN: 1572-9540
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract 197Au Mössbauer spectra have been recorded for a series of di- and trinuclear complexes of gold in different oxidation states and coordination numbers. While the interpretation of isomer shift and quadrupole coupling data is straightforward at least for groups of closely related compounds, the correlation of the line intensities with the molecular structure is more problematic. The results show that the relative line intensities in the spectra are largely determined by intramolecular motions. The recoil-free fractions may differ so much that stoichiometry-related structure assignments become hazardous.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02049111
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