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  • 1985-1989  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 20 (1985), S. 0 
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The aim of the present study was the quantification of proteins present in crevicular fluid obtained from individual sulci, and to this effect simplified micromethods for the quantification of individual proteins were developed. Unstimulated, resting crevicular fluid from healthy crevicular spaces (i.e. in the absence of clinically detectable inflammation) of dogs and humans was collected for 3 minutes, either in microcapillary glass tubes or with the aid of filter paper strips. Quantification of proteins was done by radial immunodiffusion without the pooling of samples or by immunoelectrophoresis, without eluting from the papers. The concentrations of albumin and fibrinogen in crevicular fluid so determined proved to be significantly lower than plasma concentrations. These results strengthen the concept that capillary dynamics in healthy gingivae are similar to those in general tissue capillary beds and that gingival fluid is a plasma transudate. However, due to ever present sub-clinical inflammation and other factors, it is improbable that fluid from even healthy sulci is a true transudate.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    European journal of applied physiology 55 (1986), S. 405-409 
    ISSN: 1439-6327
    Keywords: Cortisol ; Iron ; Maximal oxygen consumption ; Performance ; Endurance exercise
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary As very few of the competitors in a triathlon are truly specialist in more than one of the three disciplines, high levels of physical (and mental) stress may result during the course of the event. We investigated some of the physiological responses occurring in runners participating in an “Iron Man” triathlon consisting of canoeing (20 km), cycling (90 km) and running (42 km), in that sequence. Twenty-one male entrants volunteered as subjects for the study. Prior to the competition, maximal oxygen consumption ( $$\dot V_{{\text{O}}_{{\text{2max}}} }$$ ) was determined. Basal venous blood samples were collected on the day prior to the competition and post-exercise venous blood samples were collected within 5 minutes of completion of the race. Serum iron was significantly reduced from a mean basal value of 20.6 Μmol · l−1 to a mean value of 8.4 Μmol · l−1 after the race. Cortisol levels showed a 3 fold increase after the race. Gross $$\dot V_{{\text{O}}_{{\text{2max}}} }$$ (l · min−1) and mass standardised $$\dot V_{{\text{O}}_{{\text{2max}}} }$$ (ml · min−1 · kg−1) were both negatively correlated to cortisol levels after the race (p〈0.05). Total performance time was not related to gross $$\dot V_{{\text{O}}_{{\text{2max}}} }$$ (l · min−1) but was well correlated to mass corrected $$\dot V_{{\text{O}}_{{\text{2max}}} }$$ (ml · min−1 · kg−1). The marked fall in serum iron may have been related to heavy sweating or prelatent iron deficiency. Chronic iron deficiency (without frank anaemia) can impair physical performance, although we were unable to show any significant correlation between serum iron level after the race and time taken to complete the event. The subjects with a lower $$\dot V_{{\text{O}}_{{\text{2max}}} }$$ (ml · min−1 · kg−1) had a higher cortisol concentration. In spite of a longer performance time, these subjects were apparently still exposed to greater physiological strain.
    Type of Medium: Electronic Resource
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