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  • 1
    Electronic Resource
    Electronic Resource
    Pulsed 469.4 nm hollow cathode He-Kr laser (1989)
    Springer
    Applied physics 49 (1989), S. 343-347 
    Details
    ISSN: 1432-0649
    Keywords: 42.55F ; 42.60B
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Design principles, construction and operation characteristics of a pulsed 469.4 nm He-Kr laser are described. The laser was operated in a 20 cm active length transverse hollow cathode discharge and was excited by 50 Hz repetition rate 0.1–1 ms duration square wave current pulses. Cataphoresis of Kr ions was found to play an important role in forming the laser pulse shape. By increasing the voltage a considerable increase of peak laser power occurred, the maximum power obtained was 80 mW. The sealed-off laser was operated using 400 μs, 4 A current pulses, pulsed laser power being 20 mW, average power 0.4 mW, respectively. The lifetime of the laser was 440 h, tube failure being due to gas cleanup caused by cathode sputtering.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00324184
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Small RNAs of Tetrahymena thermophila (1987)
    Springer
    Molecular biology reports 12 (1987), S. 13-19 
    Details
    ISSN: 1573-4978
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Highly purified nuclear and cytoplasmic RNAs were obtained from Tetrahymena thermophila BVII containing only a minimal amount of cross-contamination. In the nuclear RNA fraction we have detected at least 6 distinct snRNAs. Some of the RNA species showed microheterogeneity. SnRNAs of Tetrahymena thermophila are very similar to rat snRNAs, as far as length is concerned. Our cytoplasmic small RNA fraction contained two RNAs, 7S and T7, reported recently (18) as nuclear, particularly nucleolar RNAs. Moreover, we could detect only one cytoplasmic small RNA species Tc1, Tc2 was not observed. Neither the nuclear nor the cytoplasmic small RNA species are degradation products of ribosomal RNA as was shown by Northern blotting and following hybridization with pGY17 containing the entire transcribed region of the ribosomal DNA of Tetrahymena thermophila.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00580645
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Coexpression of opsin- and VIP-like-immunoreactivity in CSF-contacting neurons of the avian brain (1988)
    Springer
    Cell & tissue research 253 (1988), S. 189-198 
    Details
    ISSN: 1432-0878
    Keywords: Cerebrospinal fluid-contacting neurons ; Monoclonal antibodies ; Opsin ; VIP ; Extraocular photoreceptors ; Ring dove ; Coturnix coturnix ; Anas platyrhynchos
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cerebrospinal fluid-contacting (CSF) cells in both the septal and the tuberal areas in the brain of the ring dove are labeled by RET-P1, a monoclonal antibody to opsin that reacts with inner and outer segment membranes of rod photoreceptors in a variety of vertebrates. Immunoblot analysis of proteins from diverse brain regions, however, revealed bands of anti-RET-P1 immunoreactivity that did not correspond to opsin. Binding of RET-P1 to opsin-containing membranes, was not inhibited by membranes rich in muscarinic and β-adrenergic receptor proteins (red blood cells, heart, lung) taken from doves. RET-P1-immunoreactive CSF-contacting cells emit a dendritic process that penetrates the ependyma and ends in a knob-like terminal suspended in the ventricle. These cells also possess other processes that penetrate more or less deeply into the neuropil. Additionally, a band of labeled fibers occurs in the external layer of the median eminence. A double-label technique demonstrated that RET-P1-positive cells coexpress VIP-like immunoreactivity. VIP-positive cells in other brain areas are not RET-P1-positive.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221754
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    Paper (German National Licenses)
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