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  • 1
    Electronic Resource
    Electronic Resource
    New experimental technique for simultaneously measuring thermal conductivity and heat capacity (1985)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 56 (1985), S. 1390-1393 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: A new technique for simultaneously measuring the thermal conductivity and heat capacity of any thin sample is described. Extensive measurements on microscope cover glass slides and on one liquid-crystal compound have been carried out. The results are in good agreement with reported values.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1138490
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  • 2
    Electronic Resource
    Electronic Resource
    Numerical models of mesophases (1988)
    [s.l.] : Nature Publishing Group
    Nature 332 (1988), S. 781-781 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SMECTIC (from the Greek for soap) is the name chosen for certain liquid phases with physical properties reminiscent of soap. Iridescent soap films, which show beautiful diffraction colours under a white light, do so mainly because the soap molecules form layered structures. Consequently, the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/332781a0
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of granulation tissue conditioned medium on the in vitro differentiation of keratinocytes (1988)
    Springer
    European archives of oto-rhino-laryngology and head & neck 245 (1988), S. 325-329 
    Details
    ISSN: 1434-4726
    Keywords: Cholesteatoma ; Terminal differentiation ; Transglutaminase ; Cell envelope
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The accumulation of desquamated keratinizing squamous epithelial cells appears to be a crucial factor in the pathogenesis of middle ear cholesteatomas. The accumulation of keratin debris is due to the proliferation and the terminal differentiation of basal keratinocytes. Since cholesteatomas are usually associated with inflammatory reactions in the middle ear cavity, we examined the effects of a granulation tissue conditioned medium on the terminal differentiation of basal keratinocytes in vitro. This conditioned medium stimulated the terminal differentiation of basal keratinocytes by showing: (a) increased incorporation of 3H-leucine into cell envelopes; (b) an increased number of SDS-insoluble cell envelopes; and (c) increased transglutaminase activity (as a marker for terminal cellular differentiation). Our present studies further suggest that inflammatory granulation tissue plays an important role in the clinical growth and development of the cholesteatoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00457987
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  • 4
    Electronic Resource
    Electronic Resource
    An immunocytochemical study of cytokeratin expression in human middle ear cholesteatoma (1989)
    Springer
    European archives of oto-rhino-laryngology and head & neck 246 (1989), S. 37-42 
    Details
    ISSN: 1434-4726
    Keywords: Cholesteatoma ; Cytokeratin ; Intermediate filaments ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An indirect immunofluorescent method with monoclonal anti-cytokeratin antibodies was used to localize various cytokeratins in human middle ear cholesteatoma. The 50 K/58 K and 56.5 K/65–67 K paired cytokeratins are markers of skin type and were found in the specimens of human middle ear cholesteatomas studied. In contrast, the 40 K and 45 K cytokeratins (markers of simple epithelia), the 48 K cytokeratin (marker of hyperproliferative epidermal disease) and the 51K cytokeratin (marker of internal organ epithelia) were absent in human middle ear cholesteatoma. These findings indicate that the pattern of cytokeratins in human middle ear cholesteatoma is similar to that of skin but is different from those of simple epithelium, internal organ epithelia, and hyperproliferative epidermal disease. These findings also support the skin type epithelial origin of cholesteatoma and strongly favor the migration theory in the genesis of cholesteatomas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00454132
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  • 5
    Electronic Resource
    Electronic Resource
    Localization of fibronectin in human middle ear cholesteatoma (1988)
    Springer
    European archives of oto-rhino-laryngology and head & neck 245 (1988), S. 160-165 
    Details
    ISSN: 1434-4726
    Keywords: Fibronectin ; Basal cell ; Cholesteatoma ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Fibronectin was localized in human cholesteatoma tissues by immunohistochemical methods. The avidin-biotin-peroxidase complex staining method was used with specific fibronectin antibody. Fibronectin appeared to be localized in the matrix of the cholesteatoma studied, particularly on the surface of the cell membranes and the nuclei of the basal cells and in connective tissue. Fibronectin was not seen in the granular layer or in the keratin area. Fibronectin was found on the surface of granulation tissue, mononuclear cells, fibroblasts and endothelial cells of blood vessels. These findings were confirmed by the immunofluorescent staining method. Our previous study showed that fibronectin induced a migration of keratinocytes, macrophages and fibroblasts demonstrated by the Boyden's chamber chemotaxis assay. Macrophages and fibroblasts were shown to produce collagenase, a bone resorption factor, in cholesteatomatous tissue. The present study showed the presence of fibronectin in the matrix of cholesteatoma and granulation tissue, suggesting that fibronectin might play an important role in the clinical development and invasive behavior of cholesteatoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00464019
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  • 6
    Electronic Resource
    Electronic Resource
    The effect of endotoxin and prostaglandin E2 on the proliferation of keratinocytes in vitro (1986)
    Springer
    European archives of oto-rhino-laryngology and head & neck 243 (1986), S. 211-214 
    Details
    ISSN: 1434-4726
    Keywords: Keratinocytes ; Endotoxin ; Prostaglandin E2 ; Infection ; Cholesteatoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Since the rate of epidermal basal cell proliferation appears to be a crucial factor in the development of acquired cholesteatoma, we studied the effect of prostaglandin E2 (PGE2) and endotoxin on the growth of keratinocytes. PGE2 and endotoxins are inflammatory mediators in chronic otitis media. Various concentrations of endotoxin and PGE2 were added to keratinocytes derived from newborn rats. The synthesis of DNA was then studied by incorporation of 3H-thymidine into the keratinocytes. We found that either endotoxin or PGE2 alone inhibited DNA synthesis by keratinocytes, while endotoxin (10 μg/ml) and PGE2 (10 ng/ml) together stimulated DNA synthesis by keratinocytes. These findings suggest that inflammatory mediators, such as endotoxin plus PGE2, in chronic otitis media stimulate the growth of epidermal basal cells of cholesteatoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00470624
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