ISSN:
1573-4943
Keywords:
nicotinic acetylcholine receptor
;
photoaffinity labeling
;
ion channel
;
conformational states
;
functional domains
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract The nicotinic acetycholine receptor was subjected to photoaffinity labeling in different conformational and functional states. The photolabel used was the ion-channel blocker [3H]-TPMP+. A procedure is described for isolating labeled δ-polypeptide chains from the receptor complex by preparative SDS-polyacrylamide gel electrophoresis. The photolabel was localized in the primary structure of the δ-chain. The site of labeling was found to be identical when photoaffinity labeling was performed in the resting, desensitized, or antagonist state, respectively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01025243
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