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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 448 (1985), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    European archives of oto-rhino-laryngology and head & neck 242 (1985), S. 63-66 
    ISSN: 1434-4726
    Keywords: cAMP ; cGMP ; Otosclerotic bone remodelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We found that the cyclic-adenosine-3′,5′-rnonophosphate (cAMP) contents of Otosclerotic human ossicles were 40–50 times greater than basal levels. Cyclic-guanosine-3′,5′-monophosphate (cGMP) levels were also found to be greater than in physiological conditions, but lower than in cortical bone. These findings suggest the extensive participation of these nucleotide coenzymes in effector cells during the process of bone resorption. This emphasizes the role of bone-resorbing cells in this process as well as the probable osteoclast progenitor role of vascular endothelial cells and their enhanced activity for differentiation. At the same time, the absence of prostaglandin F2 alpha content in the Otosclerotic bone analyzed appears to exclude cartilage remnants as a source for inducing the changes in remodelling that occur.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    European archives of oto-rhino-laryngology and head & neck 243 (1986), S. 229-232 
    ISSN: 1434-4726
    Keywords: Myosin kinase ; Otosclerosis ; Perilymph ; Organ of Corti
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The guinea pig organ of Corti contains myosin light-chain kinase (MLCK) activity. The upper and lowermost parts of the cochlea do not show significantly different activities of the enzyme, which is Ca2+ and calmodulin-dependent. Short-term noise exposure does not cause a significant change. 0.3–5 Kilodalton substances of the otosclerotic perilymph, separated by SG-25 column chromatography, inhibit the MLCK activity in in vitro organ of Corti preparations. This inhibitory action of the perilymph substances can also be observed with the purified MLCK of turkey gizzard. The activity of the enzyme can be specifically inhibited by cyclic AMP-dependent protein kinase.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0630
    Keywords: 82.50 ; 79.20
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Silicon was etched in an aqueous solution of sodium hydroxide under ir laser irradiation. Two types of lasers were used, a Nd:YAG laser with a wavelength of λ=1.06 μm and a CO2 laser with λ=10.6 μm. Small-size blind holes, through holes and reliefs were formed on a Si target, and even a special type of hole can be formed with help of a CO2 laser, namely a blind hole with a hillock in its center.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-5079
    Keywords: light-harvesting chlorophyll a/b protein ; liquid crystal ; magnetic susceptibility ; polarized fluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Displacement of particles from the purified light-harvesting chlorophyll a/b protein aggregate (LHC) was studied in magnetic fields of various strengths (0 to 1.6 T) by polarized fluorescence measurements. Macromolecular aggregates of LHC have a considerable magnetic susceptibility which enables the particles to rotate and align with their nematic axes parallel with H. As LHC is embedded in a transmembrane direction thylakoids should align perpendicular to H, the mode of alignment experimentally observed in thylakoids. The value of the magnetic susceptibility could be estimated by relating it to the integral susceptibility of the chlorophyll molecules in LHC. The fitting of this value with the field strength dependency of the fluorescence polarization ratio (FP) revealed a relationship between the LHC content of various photosynthetic membranes and their capacity for alignment, which suggested that LHC might be the torque ordering chloroplasts in a magnetic field.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of chemical crystallography 19 (1989), S. 911-918 
    ISSN: 1572-8854
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract A series of copper(II) complexes with sugar type ligands (polyalcohols, sugar acids, di- and trisaccharides) was prepared. The compositions of the complexes were determined by standard analytical methods. The results showed that carboxylato and deprotonated alcoholic hydroxy groups participate in the complex formation. The EPR spectra and magnetic susceptibility data reflected the formation of species containing isolated copper(II) centers indicating that in those molecules which contain two copper ions, these are separated by sugar ligands.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-5079
    Keywords: granum formation ; light harvesting pigment-protein complex ; thermoluminescence membrane shrinkage ; energy conservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Bean leaves grown under periodic illumination (56 cycles of 2 min light and 98 min darkness) were subsequently exposed to continuous illumination, and in connection with granum formation and accumulation of the light-harvesting pigment-protein complex thermoluminescence and light-induced shrinkage of thylakoid membranes were studied. Juvenile chloroplasts with large double sheets of thylakoids obtained under periodic light exhibited low temperature spectra of polarized fluorescence yielding fluorescence polarization (FP) values 〈 1 at 695 nm, characteristic for pheophytin emission. In the course of maturation under continuous light when normal grana appeared and the chlorophyll a/b light-harvesting photosystem II complex was incorporated into the membrane, at 695 nm the relative intensity of fluorescence dropped and FP changed to a value of 〉 1, suggesting an overlap between the emission of pheophytin and that of the chlorophyll a/b light-harvesting photosystem II complex. Thermoluminescence glow curves recorded with juvenile thylakoids displayed a relatively high proportion of emission at low temperatures (around -10°C) while with mature chloroplasts, more thermoluminescence originated from energetically deeper traps (discharged around 28°C). This means that during thylakoid development the capacity of the membrane to stabilize the separated charges increases, which might be favourable for the ultimate conservation of energy. The more extensive energization of mature thylakoids was also indicated by a light-induced decrease in the thickness of the membranes upon illumination; a change which could not be detected in juvenile thylakoids.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 146 (1988), S. 150-156 
    ISSN: 1615-6102
    Keywords: β-1,3 Glucan ; Euglena ; Freeze-etch ; Microfibril ; Paramylon ; X-ray diffraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Paramylon is the β-1,3 glucan storage carbohydrate in the euglenoid algae. Mature paramylon granules are highly crystalline, fibrillar, and have a complex substructure. X-ray diffraction was used to demonstrate that mature paramylon granules are much more crystalline than immature granules. Freeze-etch electron microscopy showed that in mature granules, the microfibrils are organized in highly ordered arrays while the microfibrils of immature granules are less organized. The data suggest that the high crystallinity of paramylon is due to higher-order aggregates of microfibrils and the interaction of water with the microfibrils. The dissolution of paramylon was recorded by darkfield videomicroscopy. In a 0.5 N NaOH solution, paramylon dissociates in a regular manner into its constituent 4 nm microfibrils, and the central region of the granule is the last remaining refractile area during the dissolution process.
    Type of Medium: Electronic Resource
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