ISSN:
1432-1017
Keywords:
Flavocytochrome b2
;
EPR
;
dipolar interaction
;
semiquinone radical
;
spin-lattice relaxation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Physics
Notes:
Abstract E.P.R. experiments and spin-lattice relaxation time measurements have been performed on Flavocytochrome b 2in the range 10 K to 100 K, to obtain information on the distance between the two prosthetic groups of the protein, flavin and heme. We have used the stabilization effect of pyruvate on the semiquinone form of the flavin, to compare the E.P.R. spectral shape and the relaxation properties of the radical when the heme is either in the ferrous form or in the ferric form. When the heme is ferric, no significant increase of the line broadening or enhancement of the relaxation rate of the radical can be detected in the range 10 K to 100 K. From these results, a minimum intercentre distance of 18 to 20 Å can be estimated.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00254891
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