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  • 1985-1989  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Growth and differentiation of human keratinocytes cultured on eye lens capsules (1985)
    Springer
    Archives of dermatological research 278 (1985), S. 120-125 
    Details
    ISSN: 1432-069X
    Keywords: Keratinocytes ; Differentiation ; Lens capsule ; Immunofluoresence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The efficiency of the outgrowth of human epidermal and hair-follicle-sheath keratinocytes was studied using three different growth substrates: plastic, type-I collagen and bovine eye lens capsules (the Epicult system). It was shown that the eye lens capsule is the best substrate, since a higher percentage of cultures showed outgrowth, and the outgrowth of epidermal keratinocytes was much more rapid. This effect is related to the faster migration (not proliferation) of cells grown on lens capsules as compared to the two other substrates. The view that lens capsules can replace the basement membrane present in vivo was supported by the finding that two basement-membrane components, i.e., laminin and fibronectin, are present on lens capsules. It was shown that, in cultures grown on lens capsules, bullous-pemphigoid antigen is restricted to the basal layer, indicating that the differentiation of these cells is comparable to that of keratinocytes grown on irradiated, non-viable pig dermis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00409218
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Differentiation of keratinocytesin vitro: a new culture vessel mimicking thein vivo situation (1985)
    Springer
    Molecular biology reports 10 (1985), S. 205-213 
    Details
    ISSN: 1573-4978
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A culture vessel consisting of two independent chambers separated only by the growth substrate is described. Cells may be cultured on both sides of the growth substrate. Culture medium and gas exposure can independently be controlled in both compartments. Human hair follicles have been used as source of keratinocytes and the bovine eye lens capsule has been explored as growth substrate. The presence of 5% CO2 in air in the lower compartment appears to have a significant effect on the morphology of the cultures. When the cultures are being exposed to air with 5% CO2, the culture medium being applied in the lower compartment, formation of corneocytes characteristic for adult stratum corneum is induced, as evidenced by light and electron microscopy. To the knowledge of the authors, this stage of differentiationin vitro has not been obtained with previously described systems. Differentiation of the lower cell layers has been characterised with specific antibodies. The possible use of the system for applied and pure scientific research is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00775977
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Proriatic hair follicle cells (1986)
    Springer
    Molecular biology reports 11 (1986), S. 247-252 
    Details
    ISSN: 1573-4978
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Calmodulin levels were measured by radioimmuno-assay in freshly isolated and cultured psoriatic human scalp hair follicle cells. The mean value ± SEM for calmodulin was 1.97±0.15 ng calmodulin μg-1 protein for 16 control subjects whereas calmodulin levels were significantly increased in psoriatic hair follicles, 2.93±0.26 ng calmodulin μg-1 protein (uninvolved skin) for 18 patients and 3.09±0.21 ng calmodulin μg-1 protein for involved skin derived hair follicles for 17 of these patients. In vitro, 3-week-old cultures of psoriatic keratinocytes contained less DNA and more calmodulin per DNA than their normal counterparts. When 6 week-old cultures of psoriatic and control hair follicle keratinocytes were compared, this difference disappeared. These results are related to the state of differentiation of these cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419604
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    Paper (German National Licenses)
    Fulltext
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