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  • 1985-1989  (7)
Materialart
Erscheinungszeitraum
Jahr
  • 1
    Digitale Medien
    Digitale Medien
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 57 (1986), S. 1880-1882 
    ISSN: 1089-7623
    Quelle: AIP Digital Archive
    Thema: Physik , Elektrotechnik, Elektronik, Nachrichtentechnik
    Notizen: Over the past three years, the total TMX-U diagnostic data base has grown to exceed 10 Mbytes from over 1300 channels; roughly triple the originally designed size. This acquisition and processing load has resulted in an experiment repetition rate exceeding 10 min per shot using the five original Hewlett–Packard HP-1000 computers with their shared disks. Our new diagnostics tend to be multichannel instruments, which, in our environment, can be more easily managed using local computers. For this purpose, we are using HP series 9000 computers for instrument control, data acquisition, and analysis. Fourteen such systems are operational with processed format output exchanged via a shared resource manager. We are presently implementing the necessary hardware and software changes to create a local area network allowing us to combine the data from these systems with our main data archive. The expansion of our diagnostic system using the parallel acquisition and processing concept allows us to increase our data base with a minimum of impact on the experimental repetition rate.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 29 (1989), S. 0 
    ISSN: 1365-3083
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: An effective method for specific depletion of mature T lymphocytes from human bone marrow mononuclear cells (BMMC) with preservation of prethymic T cells and natural killer (NK) cells is presented. The BMMC were incubated with F101.01, a monoclonal antibody recognizing an epitope of the T-cell receptor CD3 complex, and subsequently with immunomagnetic beads. Flow cytometric analysis demonstrated that mature T cells were efficiently depleted and that NK cells and prethymic T cells were preserved in the BMMC. Furthermore, T cell-mediated immune reaction as measured by thymidine incorporation after stimulation with phytohaemagglutinin was abolished, whereas NK activity as measured by 51Cr release using K562 as target cells was preserved. Recovery of colony-forming units of granulocyte macrophages was 60–70%.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    ISSN: 1365-3083
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: This study was undertaken to clarify the mechanism behind the severely decreased lymphocyte proliferative response upon stimulation with mitogens and antigens seen after allogeneic bone marrow transplantation (BMT) in man. We investigated eight BMT patients and eight controls and found that the proliferative response of patient cells was reduced both when the cells were stimulated with phytohaemagglutinin (PHA) and when they were stimulated with a combination of phorbol myristate acetate (PMA), which is an activator of protein kinase C (PKC), and the calcium ionophore A23187, which irreversibly opens for calcium transport into the cell (median relative responses were 41 and.37%, respectively). However, the PHA-induced increase in the concentration of intracellular tree calcium in post-BMT cells was not significantly different from the values found in control cells and the expression of interleukin 2 (IL-2) receptors (CD25) was only slightly decreased. However, the production of IL-2 was severely decreased in patient cells after stimulation with A23187/PMA (median 3541 units), although it was higher than in PHA-stimulated control cells (median 354 units). These results show that a direct activation of PKC by PMA combined with an increase in intracellular free calcium by A23187 cannot overcome the lymphocyte proliferation deficiency in cells from patients after allogeneic BMT. The data suggest that the defect is affecting the diacylglycerol pathway considerably more than the inositol triphosphate pathway.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 26 (1987), S. 0 
    ISSN: 1365-3083
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Lymphocytes from 21 patients sampled 1-6 months after bone marrow transplantation (BMT) were tested for functional suppressor activity against marrow-donor lymphocytes in the lymphocyte transformation test. Suppression of donor responses to allogeneic (i.e. mixed lymphocyte reaction, MLR) and antigenic stimulation by irradiated (7600 rad) post-BMT cells was observed in about two-thirds of the combinations tested (N=20 and N=9). The suppression of donor MLR and antigen responses ranged between 5-52% and 10-46%, respectively. Irradiated post-BMT cells significantly suppressed donor responses to suboptimal concentrations of phytohaemagglutinin (PHA) (median suppression: 28%; P〈0.05; N=7) and concanavalin A (Con A) (median suppression: 31%; P〈0.05; N=6). A clearly suppressive effect of post-BMT cells was observed when the ratios of CD4+/CD8+ post-BMT cells were lower than 0.5 (P〈0.01). In three experiments, the depletion of the CD8- but not of the CD4-positive subset abrogated the suppression of the donor MLR by post-BMT cells. The suppression by post-BMT cells (irradiated) of MLR and mitogen responses was comparable whether the responding cells were derived from the donor or from HLA-DR-incompatible, unrelated individuals. The proliferative capacity of post-BMT cells compared to that of donor cells was assayed in the MLR with unrelated, HLA-DR-incompatible stimulator cells. A significantly decreased proliferative capacity (median 20% of that of donor cells) was found (P〈0.01; N=16). A weak inverse correlation (P〈0.05; N=16) between the proliferative and the suppressive capacity of post-BMT cells in the MLR was observed. These findings indicate that the decreased proliferative capacity upon mitogen, antigen, and alloantigen stimulation observed in most patients within 1-6 months after BMT may be partly due to non-specific suppression by CD8+ cells.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Archives of orthopaedic and trauma surgery 104 (1985), S. 57-61 
    ISSN: 1434-3916
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Nicht-destruktive Kompressionstests mit niedriger Belastungsrate wurden an 12 menschlichen Kadavertibia-Präparaten ausgeführt, die mit einer Modulprothese aus Metall auf dem medialen oder lateralen Plateau versehen waren. Die Versuche wurden an 20 in regelmäßigen Abständen voneinander gelegenen Punkten vorgenommen. Die Reproduzierbarkeit der Messungen lag bei 16 Prozent (95 Prozent Vertrauensgrenzen). Eine statistisch signifikante Korrelation zwischen Knochensteifigkeit und Grenzbelastung an den einzelnen Punkten konnte nachgewiesen werden (r = 0.75, P 〈 0.005). Auf den medialen Plateau war die kompressive Steifigkeit am höchsten, wenn die Belastung im Zentrum der Prothese angebracht wurde, nahm aber scharf in Richtung auf die mediale und posteriore Randzone hin ab. Auf dem lateralen Plateau wurden maximale Steifigkeitswerte mehr posterior erzielt, und der Steifigkeitsgradient zur Peripherie hin war weniger ausgeprägt. Details der chirurgischen Technik und spezielle Belastungssituationen die eine ungünstige Kombination von Belastungsangriffspunkten an der Prothese erzeugen, werden diskutiert.
    Notizen: Summary Nondestructive low-strain-rate compression tests were carried out on 12 human cadaver tibiae fitted with a metal modular resurfacing component on the medial or lateral condyle. The tests were done at 20 regularly spaced points on each plateau. The reproducibility of measurement was ±16% (95% tolerance limits). A statistically significant correlation was demonstrated between stiffness and ultimate force values obtained from the same points (r = 0.75, P 〈 0.005). On the medial plateau the compressive stiffness of the system was maximal when load was applied to the center of the prosthesis, but declined sharply toward the medial and posterior margins. On the lateral plateau maximal stiffness values were obtained more posteriorly, and the stiffness gradients toward the periphery were less marked. Details of surgical technique and specific loading situations that may cause undesirable combinations of load and point of prosthetic component contact are discussed.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Springer
    Archives of orthopaedic and trauma surgery 106 (1987), S. 331-332 
    ISSN: 1434-3916
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Es wird über ein 8 Jahre altes Kind berichtet, welches sich eine Fraktur sowohl des Os hamatum als auch des Os capitatum zugezogen hat. Anhand der Literatur werden verschiedene Behandlungsmäglichkeiten sowohl konservative als auch operative diskutiert. Im vorliegenden Fall kam es unter konservativer Behandlung zur Ausheilung.
    Notizen: Summary A fracture of both the hamate and capitate bones in a child is described. The literature is reviewed with special attention to the treatment of these rare fractures.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 12 (1985), S. 181-189 
    ISSN: 1052-9306
    Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: 16 Synthetic tri- to hexapeptides and a number of unknown peptides isolated from degradations of a locust cuticle protein are analysed by fast atom bombardment mass spectrometry. The analysis includes on-probe acetylation of the sample. Molecular weight information is obtained for all the synthetic peptides and for most of the peptides of protein origin. The sequence information contained in the spectra is examined and the possibility of determining the amino acid sequence of unknown peptides is evaluated. It is concluded that sequencing of unknown peptides based on the fast atom bombardment spectra is difficult and normally only results in a partial sequence.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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