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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Environmental science & technology 23 (1989), S. 1487-1492 
    ISSN: 1520-5851
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 34 (1986), S. 1019-1024 
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food biochemistry 12 (1988), S. 0 
    ISSN: 1745-4514
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Polyphenol oxidase (EC 1.14.18.1) has been purified from Jerusalem artichoke tubers by immobilized copper affinity chromatography. The enzyme is primarily an o-dihydroxyphenol oxidase with apparent Km values of 1.9, 3.5 and 3.9 mM for chlorogenic acid, 4-methylcatechol, and catechol, respectively. Several compounds exhibited inhibitory action for the enzyme in the order of: sodium metabisulfite 〉 sodium diethyldithiocarbamate 〉 2,3-naphthalenediol 〉 thioglycollate. Multiple forms were identified by gel filtration and SDS-gradient polyacrylamide gel electrophoresis: two aggregates with apparent MW of 120 and 86 K and two monomeric subnits of 40–42 and 32–34 K, respectively. Concentration dependent association-dissociation phenomena most likely determine the multimeric state of this enzyme. While the aggregated forms exhibited specificity towards mono-, di- and polyhydroxyphenols, the low MW subunits were found active only with o-dihydroxyphenols. The isoelectric points of the various enzyme species were within the range of 4.0 to 10.0. The enzyme was found to contain appreciable amounts of associated carbohydrate material.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food biochemistry 12 (1988), S. 0 
    ISSN: 1745-4514
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: An acidic fraction of polyphenol oxidase (EC 1.14.18.1), representing the bulk activity, has been isolated from Jerusalem artichoke tubers by copper affinity chromatography, followed by DEAE-Sepharose ion-exchange chromatography. Isoelectric focusing analysis showed a cluster of activity bands (microheterogeneity) in the pH region of 4.5. The amino acid composition of the enzyme was also determined. The pH optimum for oxidation of chlorogenic acid, 4-methylcatechol and catechol was 6.0. Substrate inhibition was observed by high concentrations of chlorogenic acid. Thermal inactivation data indicated an apparent activation energy of 26.2 kcal/mol. Kinetics of inactivation, upon copper removal by KCN treatment, and reconstitution reactions revealed that the former is a much slower process than reactivation.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 14 (1987), S. 0 
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The increased susceptibility of the skin of chronically immunosuppressed individuals to viral infections and sunlight-induced malignancies suggests specific drug-induced, dysfunction of local immune mechanisms within the sun-exposed skin of these individuals. To help understand the effect of immunosuppressive therapy alone in the absence of ultraviolet light on the immune system of skin, biopsies were collected from non-sun-exposed buttock skin of control, healthy volunteers and kidney transplant recipients immunosuppressed with either azathioprine/ prednisone or cyclosporin A/prednisone and examined for incidences of T6+, and HLA-DR+ cells. No significant differences in the incidences of these 2 cell types were found (a) between control individuals and transplant recipients, (b) between transplant recipients receiving either of the immunosuppressive drug regimes, or (c) between transplant recipients who either had or had not developed skin cancer.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Freshwater biology 22 (1989), S. 0 
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SUMMARY. 1. Periphyton chlorophyll a and ash free dry weight (AFDW) were monitored in nine rivers to examine the relative importance of flows and nutrients for regulating periphyton biomass in gravel bed rivers.2. Mean annual flows in the rivers ranged from 0.94 to 169 m3 s−1, mean dissolved reactive phophorus (DRP) from 1.3 to 68 μ g 1−1, periphytic chlorophyll a from 4.6 to 73 mg m −2. and AFDW from 2.8 to 16 g m−2.3. For eight of the nine rivers NH4-N. DRP, total Kjeldahl nitrogen, total phosphorus and total suspended solids were correlated (P〈0.01) with flow, and for seven rivers conductivity was inversely correlated (P〈0.05) with flow.4. There was a hyperbolic relationship between flows and biomass, with chlorophyll a 〉100 mg m −2 and AFDW 〉20 g m−2 occurring most frequently in flows of 〈20 m3 s−1.5. Floods prevented the development of medium term (i.e. up to 2 months) maxima in biomass in five of the rivers, but maxima occurred over summer-autumn and winter-spring in the three rivers where floods were absent.6. Chlorophyll a biomass was more resistant to flooding than AFDW. Only 5993 of the forty-six recorded floods caused chlorophyll a scouring, whereas 74% of the floods caused AFDW scouring. The efficiency of scour was more influenced by the pre-flood biomass than the magnitude of the event.7. Biomass maxima were significantly correlated (P〈0.01) with mean DRP concentration during the accrual period. Overall, up to 53% of the mean annual biomass difference between rivers was explained by the mean annual DRP concentrations. However, the high correlations between nutrient concentrations and flow indicated that the nutrient data were also carrying hydrological information and that simple causal relationships between nutrients and biomass are difficult to establish in rivers.8. It is concluded that hydrological factors contribute at least equally with nutrients to the differences in periphyton biomass between the gravel-bed study rivers. They combined to explain up to 63.3% of the variance in biomass, compared with 57.6% for nutrients. It is recommended that periphyton data from gravel-bed rivers should always be viewed within the context of the flow history of the site, and not just as a function of nutrient concentrations.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    International journal of food science & technology 23 (1988), S. 0 
    ISSN: 1365-2621
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: As measured by the Brabender viscoamylograph, alkali-cooking and soaking of maize in water (nixtamalization) caused large increases in viscosity, as compared with native flour. Marked effects of cooking time on the pasting properties of nixtamal were also observed. As assessed by the blue value method, nixtamalization does not cause extensive gelatinization of the starch. This was supported by differential scanning calorimetric studies, which yielded similar gelatinization endotherms for untreated maize and nixtamal flours. However, no endotherm peak appeared in tortilla samples made from this nixtamal. Furthermore, untreated maize and nixtamal flours contained many unswollen starch granules.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of applied social psychology 17 (1987), S. 0 
    ISSN: 1559-1816
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Psychology
    Notes: Interviews with 264 callers to a relapse prevention hotline were used to explore situational determinants of coping among exsmokers facing temptations to smoke. As hypothesized, subjects were more likely to perform cognitive and behavioral coping early in abstinence. Coping was also more likely in situations where subjects had habitually smoked. Discriminant function analyses were used to predict the performance of coping from situational variables. Cognitive coping could not be predicted. Performance of behavioral coping was predictable from six situational variables which accounted for 28% of the variance in coping performance. These situational variables also accounted for the decay of behavioral coping over time. The findings imply that individual differences play a limited role in coping performance and have implications for clinical efforts to enhance smoking cessation through coping interventions.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 540 (1988), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 51 (1986), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence of various salts on stability of proteins in a fababean protein isolate were examined using differential scanning calorimetry (DSC) to monitor denaturation temperatures (Td). The ability of some salts to increase Td values, or stabilize the protein, was described as a biphasic linear relationship. The two distinct phases were attributed to two established stabilization mechanisms, electrostatic interaction and preferential hydration. Ranking of salts in terms of ability to stabilize or destabilize fababean proteins followed the lyotropic series for both major structural proteins present in the isolate. In most salt environments responses of the two proteins, legumin and vicilin, were slightly different; these differences were attributed to differences in electrostatic profiles and response to water availability for the two proteins.
    Type of Medium: Electronic Resource
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